BRENDA - Enzyme Database show
show all sequences of 1.5.4.1

Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the Omega class glutathione S-transferases

Kim, J.; Suh, H.; Kim, S.; Kim, K.; Ahn, C.; Yim, J.; Biochem. J. 398, 451-460 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene encoding enzyme is the structural gene for the eye colour mutant sepia
Drosophila melanogaster
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Drosophila melanogaster
56000
-
gel filtration
Drosophila melanogaster
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
enzyme mRNA is present at late pupal stage at a high level and in early adult, but in substantially lower level
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
head
exclusive expression in the head
Drosophila melanogaster
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
124
-
30°C, pH 7.5
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
-
671875
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
gene encoding enzyme is the structural gene for the eye colour mutant sepia
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Drosophila melanogaster
56000
-
gel filtration
Drosophila melanogaster
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
head
exclusive expression in the head
Drosophila melanogaster
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
124
-
30°C, pH 7.5
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
-
671875
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Drosophila melanogaster
Other publictions for EC 1.5.4.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
671875
Kim
Identification and characteris ...
Drosophila melanogaster
Biochem. J.
398
451-460
2006
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1
-
-
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-
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2
-
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3
-
-
-
-
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3
1
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1
1
-
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-
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1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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3
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656799
Giordano
The clot gene of Drosophila me ...
Drosophila melanogaster
Mol. Genet. Genomics
268
692-697
2003
1
-
1
-
-
-
-
-
-
-
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1
-
1
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1
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2
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1
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1
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1
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2
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437751
Wiederrecht
Purification and properties of ...
Drosophila melanogaster
J. Biol. Chem.
259
14121-14127
1984
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-
-
-
-
-
-
2
-
-
2
1
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4
-
-
1
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1
1
2
5
1
1
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2
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2
1
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1
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1
1
2
5
1
1
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