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Literature summary for 1.5.5.1 extracted from
- The METTL20 homologue from Agrobacterium tumefaciens is a dual specificity protein-lysine methyltransferase that targets ribosomal protein L7/L12 and the beta subunit of electron transfer flavoprotein (ETFbeta) (2016), J. Biol. Chem., 291, 9581-9595 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of a putative ETF-QO orthologue, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL | Agrobacterium tumefaciens |
| recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced electron-transferring flavoprotein + ubiquinone | Homo sapiens | - |
electron-transferring flavoprotein + ubiquinol | - |
? |  |
| reduced electron-transferring flavoprotein + ubiquinone | Agrobacterium tumefaciens | - |
electron-transferring flavoprotein + ubiquinol | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
electron-transferring flavoprotein + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | A9CJL8 | - |
- |
| Agrobacterium tumefaciens C58 / ATCC 33970 | A9CJL8 | - |
- |
| Homo sapiens | Q16134 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus(DE3)-RIPL by nickel affinity chromatography in presence of FAD | Homo sapiens |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus(DE3)-RIPL by nickel affinity chromatography in presence of FAD | Agrobacterium tumefaciens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | although Agrobacterium tumefaciens ETF-mediated electron transfer to 2,6-DCIP is observed with a number of different dehydrogenases, the reduction of coenzyme Q1 through the DH/ETF/ETF-QO system is only observed with dimethylglycine dehydrogenase, but not with any of the tested Agrobacterium tumefaciens acyl-CoA dehydrogenases | Agrobacterium tumefaciens | ? | - |
? | |
| additional information | the recombinant human enzyme is able to transfer electrons from the ETF protein from Agrobacterium tumefaciens strain C58 to 2,6-dichloroindophenol or coenzyme Q1 in an in vitro system | Homo sapiens | ? | - |
? | |
| additional information | although Agrobacterium tumefaciens ETF-mediated electron transfer to 2,6-DCIP is observed with a number of different dehydrogenases, the reduction of coenzyme Q1 through the DH/ETF/ETF-QO system is only observed with dimethylglycine dehydrogenase, but not with any of the tested Agrobacterium tumefaciens acyl-CoA dehydrogenases | Agrobacterium tumefaciens C58 / ATCC 33970 | ? | - |
? | |
| reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Homo sapiens | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
| reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
| reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
| reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Homo sapiens | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
| reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
| reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone | - |
Homo sapiens | electron-transferring flavoprotein + ubiquinol | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + ubiquinol | - |
? | |
| reduced electron-transferring flavoprotein + ubiquinone | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ETF-QO | - |
Homo sapiens |
| ETF-QO | - |
Agrobacterium tumefaciens |
| ETF:quinone oxidoreductase | - |
Homo sapiens |
| ETF:quinone oxidoreductase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
| 22 | - |
assay at room temperature | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
| 7.2 | - |
assay at | Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| FAD | - |
Agrobacterium tumefaciens | |
| ubiquinone | - |
Homo sapiens | |
| ubiquinone | - |
Agrobacterium tumefaciens | |
| [4Fe-4S]-center | - |
Homo sapiens | |
| [4Fe-4S]-center | - |
Agrobacterium tumefaciens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | impairment of lysine-specific reduction of ETF reduces the ability of AtETF to mediate electron transfer between ETF-dependent dehydrogenases and ETF-QO | Agrobacterium tumefaciens |
| physiological function | electron-transferring flavoprotein, ETF, mediates transfer of electrons from several mitochondrial FAD-containing dehydrogenases to the ETF:quinone oxidoreductase, ETF-QO, leading to reduction of the quinone pool of the mitochondrial respiratory chain | Homo sapiens |
| physiological function | electron-transferring flavoprotein, ETF, mediates transfer of electrons from several mitochondrial FAD-containing dehydrogenases to the ETF:quinone oxidoreductase, ETF-QO, leading to reduction of the quinone pool of the mitochondrial respiratory chain | Agrobacterium tumefaciens |
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Release 2023.1 (January 2023)
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