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Literature summary for 1.5.8.2 extracted from
- Differential coupling through Val-344 and Tyr-442 of trimethylamine dehydrogenase in electron transfer reactions with ferricenium ions and electron transferring flavoprotein (2000), Biochemistry, 39, 9188-9200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methylophilus methylotrophus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Methylophilus methylotrophus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V344I | increase of Km-value, decrease of turnover | Methylophilus methylotrophus |
| Y442G | - |
Methylophilus methylotrophus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0148 | - |
trimethylamine | wild-type form, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus |  |
| 0.0242 | - |
trimethylamine | mutant V344I, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
| 0.0659 | - |
trimethylamine | mutant Y442G, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 4Fe-4S-center | Methylophilus methylotrophus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
| trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus W3A1 | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus methylotrophus | - |
- |
- |
| Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Methylophilus methylotrophus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
| trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
trimethylamine | mutant Y442G, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
| 6.7 | - |
trimethylamine | mutant V344I, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
| 16.8 | - |
trimethylamine | electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Methylophilus methylotrophus | |
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