Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pseudomonas putida | |
additional information | not inhibited by: Ba2+, Ca2+, Fe3+, Li+, Zn2+, dithiothreitol, 2-mercaptoethanol, iodoacetate; not Mn2+, Fe2+, phenylmethane sulfonylfluoride, dinitrofluorobenzene, tetranitromethane, 5,5`-dithiobis-(2-nitrobenzoate) | Pseudomonas putida | |
PCMB | 2 mM, 59% loss of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
phenazine methosulfate | - |
Pseudomonas putida | |
2 | - |
potassium ferricyanide | - |
Pseudomonas putida | |
11 | - |
N-methyl-L-valine | - |
Pseudomonas putida | |
20 | - |
N-Methyl-L-leucine | - |
Pseudomonas putida | |
29 | - |
sarcosine | - |
Pseudomonas putida | |
69 | - |
N-Methylalanine | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
4 * 42500, SDS-PAGE | Pseudomonas putida |
170000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + acceptor + H2O | Pseudomonas putida | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | Corynebacterium sp. | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Pseudomonas putida | - |
var narensis C83 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-4°C, pH 7.5, 20% sucrose solution, 3 week, 20% loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methyl-L-alanine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide | Pseudomonas putida | L-alanine + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-leucine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide | Pseudomonas putida | L-leucine + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-valine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide | Pseudomonas putida | L-valine + formaldehyde + reduced acceptor | - |
? | |
N-methylglycine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide | Pseudomonas putida | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Pseudomonas putida | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Corynebacterium sp. | glycine + formaldehyde + reduced acceptor | - |
? | |
sarcosine + H2O + phenazine methosulfate | - |
Pseudomonas putida | glycine + formaldehyde + reduced phenazine methosulfate | - |
r | |
sarcosine + H2O + potassium ferricyanide | - |
Pseudomonas putida | glycine + formaldehyde + potassium ferrocyanide | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42500, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | sarcosine + phenazine methosulfate | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
not active below, sarcosine + phenazine methosulfate | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | at 20°C for 20 h | Pseudomonas putida |