Literature summary for 1.5.98.1 extracted from

te Broemmelstroet, B.W.; Hensgens, C.M.H.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
Purification and characterization of coenzyme F420-dependent 5,10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautothrophicum strain DELTAH (1991), Biochim. Biophys. Acta, 1073, 77-84.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.033	-	5,10-methylenetetrahydromethanopterin	-	Methanothermobacter thermautotrophicus
0.065	-	coenzyme F420	-	Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cs+	monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+	Methanothermobacter thermautotrophicus
K+	monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+	Methanothermobacter thermautotrophicus
Li+	monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+	Methanothermobacter thermautotrophicus
Na+	monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+	Methanothermobacter thermautotrophicus
Na+	3 M NaCl stimulates 5fold	Methanothermobacter thermautotrophicus
NH4+	monovalent cations stimulate in the order K+ less than Cs+ less than Na+ less than NH4+ less than Li+	Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	6 * 36000, SDS-PAGE	Methanothermobacter thermautotrophicus

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-
Methanothermobacter thermautotrophicus DELTAH	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanothermobacter thermautotrophicus

Reaction

Reaction	Comment	Organism	Reaction ID
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	ternary complex mechanism	Methanothermobacter thermautotrophicus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
736	-	-	Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability	Organism
4°C, 0.5 mM 3-[(3-cholamidopropyl)dimethylammonio]1-propanesulfonate, stable for more than 6 months	Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	r	Methanothermobacter thermautotrophicus	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	absolutely dependent on coenzyme F420	Methanothermobacter thermautotrophicus	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	r	Methanothermobacter thermautotrophicus DELTAH	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420	absolutely dependent on coenzyme F420	Methanothermobacter thermautotrophicus DELTAH	5,10-methenyltetrahydromethanopterin + reduced coenzyme F420	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 36000, SDS-PAGE	Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	oxidation of 5,10-methylenetetrahydromethanopterin	Methanothermobacter thermautotrophicus

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme F420	required	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)