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Literature summary for 1.5.98.1 extracted from

  • Klein, A.R.; Koch, J.; Stetter, K.O.; Thauer, R.K.
    Two N5,N10-methylenetetrahydromethanopterin dehydrogenases in the extreme thermophile Methanopyrus kandleri: characterization of the coenzyme F420-dependent enzyme (1993), Arch. Microbiol., 160, 186-192.
    View publication on PubMed
Search for more literature for 1.5.98.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 coenzyme F420
-
 Methanopyrus kandleri
0.08
-
 5,10-methylenetetrahydromethanopterin
-
 Methanopyrus kandleri

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 very high salt concentration are required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri
K+ very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri
Na+ very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 8 * 36000, SDS-PAGE Methanopyrus kandleri
300000
-
 gel filtration Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri
-
-
-

Oxidation Stability

Oxidation Stability Organism
moderately stable against inactivation by air. 50% loss of activity within 12 h at 4°C under aerobic conditions Methanopyrus kandleri

Purification (Commentary)

Purification (Comment) Organism
-
 Methanopyrus kandleri

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 ternary complex mechanism Methanopyrus kandleri
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1320
-
-
 Methanopyrus kandleri

Storage Stability

Storage Stability Organism
-20°C, anaerobically stored, 50% loss of activity after several weeks Methanopyrus kandleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 36000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Methanopyrus kandleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 stable for 60 min in 50 mM potassium phosphate buffer, pH 6.8. Less than 50% loss of activity after 1 h in deionized water Methanopyrus kandleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6.5
-
 Methanopyrus kandleri

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 required Methanopyrus kandleri