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Literature summary for 1.5.98.1 extracted from

  • Klein, A.R.; Thauer, R.K.
    Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri (1997), Eur. J. Biochem., 245, 386-391.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 stimulates Methanopyrus kandleri

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanopyrus kandleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
5,10-methylenetetrahydromethanopterin enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication Methanopyrus kandleri
0.03
-
5,10-methylenetetrahydromethanopterin recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication Methanopyrus kandleri
0.04
-
oxidized coenzyme F420 recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication Methanopyrus kandleri
0.08
-
oxidized coenzyme F420 enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31383
-
x * 31383, calculated from sequence Methanopyrus kandleri
34000
-
x * 34000, enzyme from Methanopyrus kandleri Methanopyrus kandleri
36000
-
x * 36000, recombinant enzyme expressed in Escherichia coli Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri P94951
-
-
Methanopyrus kandleri DSM 6324 P94951
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanopyrus kandleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
Methanopyrus kandleri DSM 6324 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
? x * 31383, calculated from sequence Methanopyrus kandleri
? x * 34000, enzyme from Methanopyrus kandleri Methanopyrus kandleri
? x * 36000, recombinant enzyme expressed in Escherichia coli Methanopyrus kandleri

Synonyms

Synonyms Comment Organism
coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase
-
Methanopyrus kandleri
MTD
-
Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Methanopyrus kandleri
70 80
-
Methanopyrus kandleri