KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus | |
0.3 | - |
N5,N10-methylenetetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
38000 | - |
8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
150000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
300000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | Methanothermobacter thermautotrophicus | - |
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r | |
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
163 | - |
- |
Methanothermobacter thermautotrophicus |
290 | - |
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus | 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r | |
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
tetramer | 4 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
inactivation after a few min, increased thermostability when salts are added | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Methanothermobacter thermautotrophicus |
7 | - |
- |
Methanothermobacter thermautotrophicus |