Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | isozymes TaCKX5a/b, TaCKX6a, and TaCKX2a expression are induced by pathogen infection, i.e. by Fusarium sp. or Puccinia sp. | Triticum aestivum |
Cloned (Comment) | Organism |
---|---|
gene HvCKX1, DNA sequence determination and analysis | Hordeum vulgare |
gene HvCKX2, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system | Hordeum vulgare |
gene HvCKX3, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of HvCKX3 in transgenic tobacco plants does not lead to increased enzyme activity or an altered phenotype | Hordeum vulgare |
additional information | transgenic tobacco plants overexpressing HvCKX2 show a very strong phenotype of cytokinin deficiency with shorter interndes, highly increased formation of roots, and a dwarf growth habit, and died without producing seeds | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Hordeum vulgare | 5739 | - |
vacuole | - |
Hordeum vulgare | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58100 | - |
x * 58100, HvCKX3, amino acid sequence calculation | Hordeum vulgare |
58800 | - |
x * 58800, HvCKX2, amino acid sequence calculation | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
cv. Luxor, gene HvCKX7, genomic DNA, barley contains at least 7 different gene homologues, gene HvCKX7, genomic DNA | - |
Hordeum vulgare | Q84U27 | cv. Luxor, gene HvCKX3, barley contains at least 7 different gene homologues | - |
Hordeum vulgare | Q8H6F6 | cv. Luxor, gene HvCKX2, barley contains at least 7 different gene homologues | - |
Hordeum vulgare | Q94KI4 | cv. Luxor, gene HvCKX1, barley contains at least 7 different gene homologues | - |
Triticum aestivum | Q94KI5 | cv. Samantha, gene TaCKX1, wheat contains at least 8 different gene homologues | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme amino acid sequence contains 5 potential glycosylation sites | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Hordeum vulgare | - |
caryopsis | - |
Hordeum vulgare | - |
kernel | - |
Hordeum vulgare | - |
kernel | developing, TaCKX6a | Triticum aestivum | - |
leaf | young | Hordeum vulgare | - |
leaf | young and adult | Hordeum vulgare | - |
leaf | Puccinia-infected: TaCKX2a | Triticum aestivum | - |
additional information | tissue specific and developmental expression of barley isozymes, overview | Hordeum vulgare | - |
additional information | tissue specific expression of wheat isozymes | Triticum aestivum | - |
root | young, TaCKX7a | Triticum aestivum | - |
seed | TaCKX1, TaCKX2b, developing, TaCKX6a/b | Triticum aestivum | - |
seedling | - |
Hordeum vulgare | - |
seedling | TaCKX1 in drought-stressed seedlings, TaCKX3 | Triticum aestivum | - |
spike | Fusarium-infected: TaCKX5a/b and TaCKX6a | Triticum aestivum | - |
spikelet | at early flowering: TaCKX7b and TaCKX8, at late flowering: TaCKX4 | Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytokinin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Hordeum vulgare | ? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
? | |
cytokinin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Hordeum vulgare | ? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
? | |
isopentenyladenine + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
isopentenyladenine 9-glucoside + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | adenine 9-glucoside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
isopentenyladenosine + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
isopentenyladenosine monophosphate + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | adenosine monophosphate + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | tissue and isozyme-specific substrate specificity | Hordeum vulgare | ? | - |
? | |
additional information | tissue and isozyme-specific substrate specificity, recombinant HvCKX2 prefers cytokinin ribosides as substrates | Hordeum vulgare | ? | - |
? | |
trans-zeatin + 2,6-dichlorophenolindophenol | cis-zeatin is utilized only by some tissue-specific forms but not by HvCKX2 | Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
trans-zeatin O-glucoside + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
trans-zeatin riboside + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
trans-zeatin riboside O-glucoside + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
trans-zeatin ribotide + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
trans-zeatin-9-glucoside + 2,6-dichlorophenolindophenol | - |
Hordeum vulgare | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58100, HvCKX3, amino acid sequence calculation | Hordeum vulgare |
? | x * 58800, HvCKX2, amino acid sequence calculation | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
CKX | - |
Hordeum vulgare |
cytokinin oxidase/dehydrogenase | - |
Hordeum vulgare |
cytokinin oxidase/dehydrogenase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 | Hordeum vulgare |
4.5 | - |
recombinant HvCKX2 expressed in tobacco, native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 | Hordeum vulgare |
7 | 7.5 | native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding motif | Hordeum vulgare | |
FAD | binding motif | Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | sequence calculation | - |
6 |
Hordeum vulgare | sequence calculation | - |
7.1 |