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Literature summary for 1.5.99.12 extracted from
- Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species (2003), J. Plant Res., 116, 241-252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cytokinins induce enzyme activity, predominantly of the glycosylated isozyme | Nicotiana tabacum | |
| additional information | enzyme is induced by cytokinins | Dendrobium hybrid cultivar |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 5 distinct genes OsCKX1-OsCKX5, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Oryza sativa |
| 7 distinct genes, AtCKX1-AtCKX7, DNA and amino acid sequence determination and analysis, functional expression of AtCKX2 and AtCKX4 in Pichia pastoris, Physcomitrella patens, and Saccharomyces cerevisiae, the recombinant enzymes are located in protoplasts or are secreted to the medium, overview, phylogenetic analysis | Arabidopsis thaliana |
| gene DsCKX1, DNA and amino acid sequence determination and analysis, analysis of expression, regulation, phylogenetic analysis | Dendrobium hybrid cultivar |
| gene FasV of RfCKX1, expression as polycistronic mRNA, fas operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Rhodococcus fascians |
| gene HvCKX2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Hordeum vulgare |
| gene NsCKX1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Nostoc sp. |
| gene ZmCKX1, DNA and amino acid sequence determination and analysis, functional expression in Pichia pastoris, Physcomitrella patens, and Saccharomyces cerevisiae, phylogenetic analysis | Zea mays |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | phenotypic effects of enzyme overexpression in transgenic tobacco, overview | Nicotiana tabacum |
| additional information | transgenic tobacco plants overexpressing ArCKX1, AtCKX5, or AtCKX6 shows increased enzyme activity and reduced cytokinin content phenotype, phenotypic effects of enzyme overexpression in transgenic Arabidopsis plants, overview | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphenylurea | competitive | Zea mays |  |
| additional information | enzyme is strongly inhibited by flavin-protein oxidoreductase inhibitors | Zea mays | |
| N6-isopentenyl-N-methylaminopurine | structural analogue of isopentenyladenine | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
N6-dimethylallyladenine | pH 7.0 | Zea mays | |
| 0.006 | - |
trans-zeatin | pH 9.0 | Arabidopsis thaliana | |
| 0.011 | - |
trans-zeatin riboside | pH 7.0 | Zea mays | |
| 0.014 | - |
trans-zeatin | pH 7.0 | Zea mays | |
| 0.015 | - |
N6-dimethylallyladenine | pH 9.0 | Arabidopsis thaliana | |
| 0.04 | - |
N6-dimethylallyladenine | pH 6.5 | Arabidopsis thaliana | |
| 0.046 | - |
cis-zeatin | pH 7.0 | Zea mays | |
| 0.054 | - |
N6-dimethylallyladenine | pH 7.0 | Zea mays | |
| 0.06 | - |
trans-zeatin | pH 6.5 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion pathway | Dendrobium hybrid cultivar | - |
- |
| extracellular | secretion pathway | Zea mays | - |
- |
| extracellular | secretion pathway, enzymes AtCKX2, and AtCKX3-AtCKX6 | Arabidopsis thaliana | - |
- |
| extracellular | secretion pathway, enzymes OsCKX1, and OsCKX3-OsCKX5 | Oryza sativa | - |
- |
| extracellular | secretion pathway, the glycosylated isozyme accumulates preferentially in the culture medium | Nicotiana tabacum | - |
- |
| extracellular | secretory pathway | Hordeum vulgare | - |
- |
| mitochondrion | enzyme OsCKX2 | Oryza sativa | 5739 | - |
| mitochondrion | enzymes AtCKX1 and AtCKX3 | Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47900 | - |
x * 47900, amino acid sequence calculation | Rhodococcus fascians |
| 49800 | - |
x * 49800, amino acid sequence calculation | Nostoc sp. |
| 56000 | - |
x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation | Oryza sativa |
| 56500 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 57200 | - |
x * 57200, amino acid sequence calculation | Zea mays |
| 57400 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 57900 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 58000 | - |
x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation | Oryza sativa |
| 58100 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 58400 | - |
x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation | Oryza sativa |
| 58800 | - |
x * 58800, amino acid sequence calculation | Hordeum vulgare |
| 59100 | - |
x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation | Oryza sativa |
| 59400 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 59900 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
| 60400 | - |
x * 60400, DsCKX1, amino acid sequence calculation | Dendrobium hybrid cultivar |
| 64900 | - |
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis/trans-zeatin + FAD + H2O | Zea mays | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| additional information | Dendrobium hybrid cultivar | analysis of enzyme activity regulation | ? | - |
? | |
| additional information | Nostoc sp. | cytokinins stimulate pigment formation and nitrogen fixation | ? | - |
? | |
| additional information | Dictyostelium discoideum | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes | ? | - |
? | |
| additional information | Rhodococcus fascians | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes during infection, cytokinins are involved in pathogenesis | ? | - |
? | |
| additional information | Nicotiana tabacum | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| additional information | Oryza sativa | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| additional information | Phaseolus sp. | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| additional information | Hordeum vulgare | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| additional information | Arabidopsis thaliana | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| additional information | Zea mays | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | ? | - |
? | |
| N6-dimethylallyladenine + FAD + H2O | Nostoc sp. | - |
adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Dendrobium hybrid cultivar | - |
adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Nicotiana tabacum | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Dictyostelium discoideum | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Oryza sativa | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Phaseolus sp. | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Hordeum vulgare | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Arabidopsis thaliana | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Zea mays | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | Rhodococcus fascians | enzyme catalyzes the irreversible degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| trans-zeatin + FAD + H2O | Arabidopsis thaliana | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Nostoc sp. | - |
adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Dendrobium hybrid cultivar | - |
adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Nicotiana tabacum | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Dictyostelium discoideum | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Oryza sativa | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Phaseolus sp. | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Hordeum vulgare | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | Rhodococcus fascians | enzyme catalyzes the irreversible degradation of cytokinins | adenine + ? + FADH2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FUJ3 | - |
- |
| Dendrobium hybrid cultivar | Q9FE45 | gene DsCKX1 or DSCKX1 | - |
| Dictyostelium discoideum | - |
- |
- |
| Hordeum vulgare | Q8H6F6 | gene HvCKX2 | - |
| Nicotiana tabacum | - |
2 isozymes: 1 glycosylated, 1 non-glycosylated | - |
| no activity in Physcomitrella patens | - |
- |
- |
| no activity in Pichia pastoris | - |
- |
- |
| no activity in Prochlorococcus marinus | - |
- |
- |
| no activity in Raphanus sativus | - |
- |
- |
| no activity in Saccharomyces cerevisiae | - |
- |
- |
| no activity in Synechocystis sp. | - |
- |
- |
| no activity in Synechocystis sp. PCC 6803 | - |
- |
- |
| Nostoc sp. | - |
i.e. Anabaena sp., PCC 7120, gene NsCKX1 | - |
| Oryza sativa | - |
5 distinct genes OsCKX1-OsCKX5 | - |
| Phaseolus sp. | - |
2 isozymes: 1 glycosylated, 1 non-glycosylated | - |
| Rhodococcus fascians | - |
phytopathogen causing the witches broom disease, gene FasV of RfCKX1 | - |
| Zea mays | Q9T0N8 | gene ZmCKX1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 2 isozymes: 1 glycosylated, 1 non-glycosylated | Nicotiana tabacum |
| glycoprotein | 2 isozymes: 1 glycosylated, 1 non-glycosylated | Phaseolus sp. |
| glycoprotein | enzyme amino acid sequence contains 1 putative glycosylation site | Nostoc sp. |
| glycoprotein | enzyme amino acid sequence contains 2 putative glycosylation sites | Dendrobium hybrid cultivar |
| glycoprotein | enzyme amino acid sequence contains 2 putative glycosylation sites | Rhodococcus fascians |
| glycoprotein | enzyme amino acid sequence contains 5 putative glycosylation sites | Hordeum vulgare |
| glycoprotein | enzyme amino acid sequence contains 5-8 putative glycosylation sites | Zea mays |
| glycoprotein | enzyme amino acid sequences encoded by the 5 distinct genes in rice contain 1-6 putative glycosylation sites | Oryza sativa |
| glycoprotein | enzyme amino acid sequences encoded by the 7 distinct genes in Arabidopsis contain 3 to 8 putative glycosylation sites, overview | Arabidopsis thaliana |
| no glycoprotein | 2 isozymes: 1 glycosylated, 1 non-glycosylated | Nicotiana tabacum |
| no glycoprotein | 2 isozymes: 1 glycosylated, 1 non-glycosylated | Phaseolus sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor | reaction might also be catalyzed by a different enzyme in Dictyostelium discoideum, since no related sequence has been found | Dictyostelium discoideum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Nicotiana tabacum | - |
| kernel | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis/trans-zeatin + FAD + H2O | - |
Zea mays | adenine + ? + FADH2 | - |
ir | |
| cis/trans-zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Zea mays | adenine + ? + FADH2 | - |
ir | |
| cis/trans-zeatin riboside + FAD + H2O | - |
Zea mays | adenine + ? + FADH2 | - |
ir | |
| additional information | analysis of enzyme activity regulation | Dendrobium hybrid cultivar | ? | - |
? | |
| additional information | cytokinins stimulate pigment formation and nitrogen fixation | Nostoc sp. | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes | Dictyostelium discoideum | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes during infection, cytokinins are involved in pathogenesis | Rhodococcus fascians | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Nicotiana tabacum | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Oryza sativa | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Phaseolus sp. | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Hordeum vulgare | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development | Zea mays | ? | - |
? | |
| additional information | the free bases and their ribosides are the preferred substrates | Arabidopsis thaliana | ? | - |
? | |
| N6-dimethylallyladenine + FAD + H2O | - |
Nostoc sp. | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | - |
Dendrobium hybrid cultivar | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Nicotiana tabacum | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Dictyostelium discoideum | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Oryza sativa | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Phaseolus sp. | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Hordeum vulgare | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Arabidopsis thaliana | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Rhodococcus fascians | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Nicotiana tabacum | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Dictyostelium discoideum | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Oryza sativa | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Nostoc sp. | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Phaseolus sp. | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Arabidopsis thaliana | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenine + FAD + H2O | i.e. isopentenyladenine | Rhodococcus fascians | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| N6-dimethylallyladenosine + FAD + H2O | i.e. isopentenyladenosine | Zea mays | adenosine + 3-methylbut-2-enal + FADH2 | - |
ir | |
| trans-zeatin + FAD + H2O | - |
Arabidopsis thaliana | adenine + ? + FADH2 | - |
ir | |
| trans-zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Arabidopsis thaliana | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Nicotiana tabacum | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Dictyostelium discoideum | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Oryza sativa | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Nostoc sp. | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Phaseolus sp. | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Dendrobium hybrid cultivar | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | - |
Rhodococcus fascians | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Nicotiana tabacum | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Dictyostelium discoideum | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Oryza sativa | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Phaseolus sp. | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Hordeum vulgare | adenine + ? + FADH2 | - |
ir | |
| zeatin + FAD + H2O | enzyme catalyzes the irreversible degradation of cytokinins | Rhodococcus fascians | adenine + ? + FADH2 | - |
ir | |
| zeatin riboside + 2,6-dichlorophenolindophenol | - |
Zea mays | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47900, amino acid sequence calculation | Rhodococcus fascians |
| ? | x * 49800, amino acid sequence calculation | Nostoc sp. |
| ? | x * 57200, amino acid sequence calculation | Zea mays |
| ? | x * 58800, amino acid sequence calculation | Hordeum vulgare |
| ? | x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation | Oryza sativa |
| ? | x * 60400, DsCKX1, amino acid sequence calculation | Dendrobium hybrid cultivar |
| ? | x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CKX | - |
Nicotiana tabacum |
| CKX | - |
Dictyostelium discoideum |
| CKX | - |
Oryza sativa |
| CKX | - |
Nostoc sp. |
| CKX | - |
Phaseolus sp. |
| CKX | - |
Hordeum vulgare |
| CKX | - |
Dendrobium hybrid cultivar |
| CKX | - |
Arabidopsis thaliana |
| CKX | - |
Zea mays |
| CKX | - |
Rhodococcus fascians |
| cytokinin oxidase | - |
Zea mays |
| cytokinin oxidase/dehydrogenase | - |
Nicotiana tabacum |
| cytokinin oxidase/dehydrogenase | - |
Dictyostelium discoideum |
| cytokinin oxidase/dehydrogenase | - |
Oryza sativa |
| cytokinin oxidase/dehydrogenase | - |
Nostoc sp. |
| cytokinin oxidase/dehydrogenase | - |
Phaseolus sp. |
| cytokinin oxidase/dehydrogenase | - |
Hordeum vulgare |
| cytokinin oxidase/dehydrogenase | - |
Dendrobium hybrid cultivar |
| cytokinin oxidase/dehydrogenase | - |
Arabidopsis thaliana |
| cytokinin oxidase/dehydrogenase | - |
Zea mays |
| cytokinin oxidase/dehydrogenase | - |
Rhodococcus fascians |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Arabidopsis thaliana |
| additional information | - |
the 2 isozymes show largely different different pH-optima | Phaseolus sp. |
| additional information | - |
the 2 isozymes show largely different pH-optima | Nicotiana tabacum |
| 7 | - |
assay at | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Nicotiana tabacum | |
| FAD | - |
Oryza sativa | |
| FAD | - |
Nostoc sp. | |
| FAD | - |
Phaseolus sp. | |
| FAD | - |
Hordeum vulgare | |
| FAD | - |
Dendrobium hybrid cultivar | |
| FAD | - |
Arabidopsis thaliana | |
| FAD | - |
Rhodococcus fascians | |
| FAD | binding site comprising amino acid residues 69-105, covalent binding through a histidine residue | Zea mays | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | 0.008 | diphenylurea | recombinant enyme | Zea mays | |
| 0.012 | - |
N6-isopentenyl-N-methylaminopurine | recombinant enyme | Zea mays | |
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Release 2023.1 (January 2023)
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