Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cytokinin-induced up-regulation of enzyme activity, e.g. by N6-benzylaminopurine application on the plant leaf or callus surface leads to about 2.5fold increased activity after 4 h, overview | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic tobacco plants conditionally overexpressing isopentenyltransferase IPT, tetracycline-induced derepressing of the IPT transcription, results in enhanced cytokinin oxidase activity | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme activity dynamics in relation to glycosylation level | Nicotiana tabacum | |
0.001 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by tetracycline, pH 6.0, 37°C | Nicotiana tabacum | |
0.0015 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by tetracycline, pH 6.0, 37°C | Nicotiana tabacum | |
0.0017 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by N6-benzylaminopurine, pH 6.0, 37°C | Nicotiana tabacum | |
0.0022 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract, pH 6.0, 37°C | Nicotiana tabacum | |
0.0023 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by N6-benzylaminopurine, pH 6.0, 37°C | Nicotiana tabacum | |
0.0025 | - |
N6-(DELTA2-isopentenyl)adenine | transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium, pH 6.0, 37°C | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
cv. Wisconsin 38, wild-type, and transgenic tobacco conditionally overexpressing isopentenyltransferase IPT | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme binds with moderate affinity to concanavalin A, glycosylation is influenced by cytokinins and has a regulatory role for the enzyme activity, glycosylation changes the enzyme secretion level | Nicotiana tabacum |
Purification (Comment) | Organism |
---|---|
partial from leaves and calli of wild-type and transgenic plants | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Nicotiana tabacum | - |
cell suspension culture | - |
Nicotiana tabacum | - |
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium, not induced | Nicotiana tabacum |
0.0003 | - |
wild-type plant, cell extract and cell culture medium, not induced or tetracycline-treated cells | Nicotiana tabacum |
0.0004 | - |
transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, not induced cells | Nicotiana tabacum |
0.002 | - |
wild-type plant and transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, N6-benzylaminopurine-induced | Nicotiana tabacum |
0.004 | - |
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of N6-benzylaminopurine-induced cells | Nicotiana tabacum |
0.005 | - |
wild-type plant, cell culture medium of N6-benzylaminopurine-induced cells | Nicotiana tabacum |
0.0065 | - |
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of tetracycline-treated cells | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway | Nicotiana tabacum | ? | - |
? | |
N6-(DELTA2-isopentenyl)adenine + acceptor + H2O | - |
Nicotiana tabacum | adenine + 3-methylbut-2-enal + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CKX | - |
Nicotiana tabacum |
cytokinin oxidase | - |
Nicotiana tabacum |
More | see also EC 1.4.3.18 | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
secreted enzyme in cell culture medium | Nicotiana tabacum |
8.5 | - |
enzyme in cells | Nicotiana tabacum |