Application | Comment | Organism |
---|---|---|
analysis | development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane. The D-Pro DH/agar immobilized electrode is applied to determination of D-amino acids in a human urine sample | Pyrobaculum islandicum |
General Stability | Organism |
---|---|
enzyme activity in the D-Pro dehydrogenase/agar immobilized electrode is retained over 90% for 80 days | Pyrobaculum islandicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
D-proline | - |
Pyrobaculum islandicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated to | Pyrobaculum islandicum | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + oxidized 2,6-dichloroindophenol + H2O | 9% of the activity with D-proline | Pyrobaculum islandicum | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-aspartate + oxidized 2,6-dichloroindophenol + H2O | 7% of the activity with D-proline | Pyrobaculum islandicum | oxaloacetate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-histidine + oxidized 2,6-dichloroindophenol + H2O | 29% of the activity with D-proline | Pyrobaculum islandicum | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-isoleucine + oxidized 2,6-dichloroindophenol + H2O | 46% of the activity with D-proline | Pyrobaculum islandicum | 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-leucine + oxidized 2,6-dichloroindophenol + H2O | 19% of the activity with D-proline | Pyrobaculum islandicum | 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-proline + oxidized 2,6-dichloroindophenol | - |
Pyrobaculum islandicum | DELTA1-pyrroline 2-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
D-valine + oxidized 2,6-dichloroindophenol + H2O | 18% of the activity with D-proline | Pyrobaculum islandicum | 2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-Pro DH | - |
Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrobaculum islandicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pyrobaculum islandicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Pyrobaculum islandicum |