Literature summary for 1.5.99.14 extracted from

Wang, L.; Mu, X.; Li, W.; Xu, Q.; Xu, P.; Zhang, L.; Zhang, Y.; Wu, G.
Structural, mechanistic, and functional insights into an Arthrobacter nicotinovorans molybdenum hydroxylase involved in nicotine degradation (2021), Molecules, 26, 4387 .

Search for more literature for 1.5.99.14

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of the Kdh holoenzyme, with its three subunits KdhL, KdhM, and KdhS, and their associated cofactors molybdopterin cytosine dinucleotide, two iron-sulfur clusters (Fe2S2), and flavin adenine dinucleotide, respectively. The residues Glu345, Try551, and Glu748 of KdhL participate in substrate binding, and Phe269 and Arg383 of KdhL contribute to stabilize the molybdopterin cytosine dinucleotide conformation	Paenarthrobacter nicotinovorans

Crystallization (Comment)

Organism

structure of the Kdh holoenzyme, with its three subunits KdhL, KdhM, and KdhS, and their associated cofactors molybdopterin cytosine dinucleotide, two iron-sulfur clusters (Fe2S2), and flavin adenine dinucleotide, respectively. The residues Glu345, Try551, and Glu748 of KdhL participate in substrate binding, and Phe269 and Arg383 of KdhL contribute to stabilize the molybdopterin cytosine dinucleotide conformation

Paenarthrobacter nicotinovorans

Protein Variants

Protein Variants	Comment	Organism
E345A	mutation in subunit KdhL, inactive	Paenarthrobacter nicotinovorans
E748A	mutation in subunit KdhL, inactive	Paenarthrobacter nicotinovorans
F269A	mutation in subunit KdhL, 8fold decrease in catalytic efficiency	Paenarthrobacter nicotinovorans
R383A	mutation in subunit KdhL, 2fold decrease in catalytic efficiency	Paenarthrobacter nicotinovorans
W551A	mutation in subunit KdhL, inactive	Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0125	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
0.0125	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
0.0151	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.5, 30°C	Paenarthrobacter nicotinovorans
0.0159	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
0.0214	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant R383A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
0.0223	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 7.4, 30°C	Paenarthrobacter nicotinovorans
0.0234	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 7.4, 30°C	Paenarthrobacter nicotinovorans
0.026	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant F269A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
0.0377	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 6.8, 30°C	Paenarthrobacter nicotinovorans
0.065	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 6.4, 30°C	Paenarthrobacter nicotinovorans

Organism

Organism	UniProt	Comment	Textmining
Paenarthrobacter nicotinovorans	O87681 and O87682 and Q933N0	O87681 i.e. subunit KdhA/KdhM, O87682 i.e. subunit KdhB/KdhS, Q933N0 i.e. sunbunit KdhC/KdhL	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + phenazine methosulfate + H2O	-	Paenarthrobacter nicotinovorans	1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced phenazine methosulfate	-	?

Synonyms

Synonyms	Comment	Organism
KdhA	-	Paenarthrobacter nicotinovorans
KdhB	-	Paenarthrobacter nicotinovorans
KdhC	-	Paenarthrobacter nicotinovorans
KdhL	-	Paenarthrobacter nicotinovorans
KdhM	-	Paenarthrobacter nicotinovorans
KdhS	-	Paenarthrobacter nicotinovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
2.3	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant F269A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
5.13	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 6.4, 30°C	Paenarthrobacter nicotinovorans
6.3	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant R383A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
8.92	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.5, 30°C	Paenarthrobacter nicotinovorans
9.03	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
9.03	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
9.09	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 6.8, 30°C	Paenarthrobacter nicotinovorans
9.28	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 7.4, 30°C	Paenarthrobacter nicotinovorans
9.47	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
10.58	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 7.4, 30°C	Paenarthrobacter nicotinovorans

Cofactor

Cofactor	Comment	Organism
FAD	-	Paenarthrobacter nicotinovorans
iron-sulfur centre	presence of two [Fe2-S2] centers	Paenarthrobacter nicotinovorans
molybdopterin cytosine dinucleotide	-	Paenarthrobacter nicotinovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
80	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 6.4, 30°C	Paenarthrobacter nicotinovorans
90	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant F269A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
240	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 6.8, 30°C	Paenarthrobacter nicotinovorans
300	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	mutant R383A, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
420	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 7.4, 30°C	Paenarthrobacter nicotinovorans
450	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 7.4, 30°C	Paenarthrobacter nicotinovorans
590	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.5, 30°C	Paenarthrobacter nicotinovorans
600	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, Tris/HCl, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
720	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, pH 8.0, 30°C	Paenarthrobacter nicotinovorans
720	-	1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one	wild-type, PBS, pH 8.0, 30°C	Paenarthrobacter nicotinovorans