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Literature summary for 1.5.99.4 extracted from

  • Williams, D.E.; Ding, X.; Coon, M.J.
    Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase (1990), Biochem. Biophys. Res. Commun., 166, 945-952.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
(S)-nicotine
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome olfactory and respiratory Oryctolagus cuniculus
-
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
male, adult, New Zealand White
-

Source Tissue

Source Tissue Comment Organism Textmining
nose
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0065
-
respiratory microsomes Oryctolagus cuniculus
0.0222
-
olfactory microsomes Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-nicotine + acceptor
-
Oryctolagus cuniculus (S)-nicotine delta1',5'-iminium ion + reduced acceptor
-
?
(S)-nicotine + acceptor + H2O
-
Oryctolagus cuniculus nornicotine + reduced acceptor + ?
-
?
(S)-nicotine + acceptor + H2O
-
Oryctolagus cuniculus nicotine-N-oxide + reduced acceptor
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.468
-
(S)-nicotine respiratory Oryctolagus cuniculus
0.597
-
(S)-nicotine olfactory Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 P-450 NMa monooxygenase Oryctolagus cuniculus
additional information flavoprotein Oryctolagus cuniculus