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Literature summary for 1.5.99.5 extracted from

  • Bamforth, C.W.; Large, P.J.
    Solubilization and partial purification of N-methylglutamate dehydrogenase from Pseudomonas aminovorans (1975), Biochem. Soc. Trans., 3, 1066-1069.
No PubMed abstract available
Search for more literature for 1.5.99.5

Inhibitors

Inhibitors Comment Organism Structure
deoxycholate
-
 Aminobacter aminovorans
Lubrol Px
-
 Aminobacter aminovorans
Triton X-100
-
 Aminobacter aminovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 phenazine methosulfate
-
 Aminobacter aminovorans
0.019
-
 2,6-dichlorophenolindophenol
-
 Aminobacter aminovorans
0.025
-
 N-Methyl-L-glutamate
-
 Aminobacter aminovorans
0.11
-
 horse heart cytochrome c
-
 Aminobacter aminovorans
0.46
-
 N-methyl DL-aspartate
-
 Aminobacter aminovorans
87
-
 N-methyl DL-alanine
-
 Aminobacter aminovorans
90.9
-
 sarcosine
-
 Aminobacter aminovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas sp. 16020
-
membrane
-
 Aminobacter aminovorans 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-methyl-L-glutamate + acceptor + H2O Pseudomonas sp.
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Aminobacter aminovorans
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O Pseudomonas sp. MA / M.A. / ATCC 23819
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans AM1
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Aminobacter aminovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
-
 Aminobacter aminovorans

Storage Stability

Storage Stability Organism
-15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml Aminobacter aminovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Pseudomonas sp. ?
-
 ?
additional information L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+ Aminobacter aminovorans ?
-
 ?
additional information L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+ Aminobacter aminovorans AM1 ?
-
 ?
additional information
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 ?
-
 ?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methyl-L-glutamate + acceptor + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced acceptor
-
 ?
N-methylglutamate + horse heart cytochrome c + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
 ir
N-methylglutamate + horse heart cytochrome c + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced horse heart cytochrome c
-
 ir
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Aminobacter aminovorans AM1 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
N-methylglutamate + phenazine methosulfate + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 L-glutamate + formaldehyde + reduced phenazine methosulfate
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Aminobacter aminovorans aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aminobacter aminovorans
7 8.5
-
 Pseudomonas sp.