BRENDA - Enzyme Database
show all sequences of 1.5.99.5

Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans

Bamforth, C.W.; Large, P.J.; Biochem. J. 161, 357-370 (1977)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Aminobacter aminovorans
Cu2+
total inhibition at 1 mM
Aminobacter aminovorans
formaldehyde
-
Aminobacter aminovorans
iodoacetamide
-
Aminobacter aminovorans
p-chloromercuribenzoate
-
Aminobacter aminovorans
Triton X-100
-
Aminobacter aminovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
N-Methyl-L-glutamate
-
Aminobacter aminovorans
0.21
-
2,6-dichlorophenolindophenol
-
Aminobacter aminovorans
0.46
-
N-methyl DL-aspartate
-
Aminobacter aminovorans
25
-
N-methyl L-alanine
-
Aminobacter aminovorans
33
-
N-methyl D-alanine
-
Aminobacter aminovorans
87
-
N-methyl DL-alanine
-
Aminobacter aminovorans
90.9
-
sarcosine
-
Aminobacter aminovorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aminobacter aminovorans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM stimulates activity by 33%
Aminobacter aminovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Aminobacter aminovorans
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans AM1
-
-
-
Aminobacter aminovorans N.C.I.B. 9039
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Aminobacter aminovorans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00033
-
crude extract, grown on trimethylamine N-oxide
Aminobacter aminovorans
0.0018
-
crude extract, grown on trimethylamine
Aminobacter aminovorans
0.0025
-
crude extract, grown on methylamine
Aminobacter aminovorans
0.00364
-
crude extract, grown on dimethylamine
Aminobacter aminovorans
0.0554
-
-
Aminobacter aminovorans
Storage Stability
Storage Stability
Organism
-15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml
Aminobacter aminovorans
-196°C, survives cooling with subsequent thawing
Aminobacter aminovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
40
heat-labile with half-lives of 35 min at 30°C and 5 min at 40°C
Aminobacter aminovorans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
-
Aminobacter aminovorans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
acid labile, stable at neutrality and pH values above 7.0
Aminobacter aminovorans
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b
-
Aminobacter aminovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b
-
Aminobacter aminovorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Aminobacter aminovorans
Cu2+
total inhibition at 1 mM
Aminobacter aminovorans
formaldehyde
-
Aminobacter aminovorans
iodoacetamide
-
Aminobacter aminovorans
p-chloromercuribenzoate
-
Aminobacter aminovorans
Triton X-100
-
Aminobacter aminovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
N-Methyl-L-glutamate
-
Aminobacter aminovorans
0.21
-
2,6-dichlorophenolindophenol
-
Aminobacter aminovorans
0.46
-
N-methyl DL-aspartate
-
Aminobacter aminovorans
25
-
N-methyl L-alanine
-
Aminobacter aminovorans
33
-
N-methyl D-alanine
-
Aminobacter aminovorans
87
-
N-methyl DL-alanine
-
Aminobacter aminovorans
90.9
-
sarcosine
-
Aminobacter aminovorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aminobacter aminovorans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM stimulates activity by 33%
Aminobacter aminovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Aminobacter aminovorans
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Aminobacter aminovorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00033
-
crude extract, grown on trimethylamine N-oxide
Aminobacter aminovorans
0.0018
-
crude extract, grown on trimethylamine
Aminobacter aminovorans
0.0025
-
crude extract, grown on methylamine
Aminobacter aminovorans
0.00364
-
crude extract, grown on dimethylamine
Aminobacter aminovorans
0.0554
-
-
Aminobacter aminovorans
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml
Aminobacter aminovorans
-196°C, survives cooling with subsequent thawing
Aminobacter aminovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans AM1
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
-
437745
Aminobacter aminovorans N.C.I.B. 9039
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
-
437745
Aminobacter aminovorans
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
40
heat-labile with half-lives of 35 min at 30°C and 5 min at 40°C
Aminobacter aminovorans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
-
Aminobacter aminovorans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
acid labile, stable at neutrality and pH values above 7.0
Aminobacter aminovorans
Other publictions for EC 1.5.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700286
Latypova
Genetics of the glutamate-medi ...
Methyloversatilis universalis, Methyloversatilis universalis FAM5
Mol. Microbiol.
75
426-439
2010
-
-
1
-
-
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-
-
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-
2
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5
-
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4
1
3
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1
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2
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4
1
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-
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-
-
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-
2
2
-
-
-
723999
Chen
gamma-Glutamylmethylamide is a ...
Methylocella silvestris, Methylocella silvestris BL2
Appl. Environ. Microbiol.
76
4530-4537
2010
-
-
-
-
-
-
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2
-
7
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1
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4
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2
1
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1
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2
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1
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4
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
437750
Biville
-
Physiological properties of a ...
Methylobacterium organophilum
FEMS Microbiol. Lett.
52
53-58
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437749
Janvier
-
Methylophaga marina gen nov., ...
Methylophaga marina, Methylophaga thalassica
Int. J. Syst. Bacteriol.
35
131-139
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
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2
-
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2
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437748
Boulton
-
N-Methylglutamate dehydrogenas ...
bacterium AT2, Hyphomicrobium vulgare, Pseudomonas sp., Aminobacter aminovorans, Pseudomonas methylica, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819, Hyphomicrobium vulgare ZV, Hyphomicrobium vulgare 3
J. Gen. Microbiol.
117
293-304
1980
-
-
-
-
-
-
-
2
4
-
2
7
-
18
-
-
2
-
-
-
6
-
39
2
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
4
-
2
7
-
-
-
2
-
-
6
-
39
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
437747
Bamforth
The kinetic mechanism of N-met ...
Aminobacter aminovorans
Biochem. Soc. Trans.
6
193-195
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437745
Bamforth
Solubilization, partial purifi ...
Aminobacter aminovorans, Aminobacter aminovorans AM1, Aminobacter aminovorans N.C.I.B. 9039
Biochem. J.
161
357-370
1977
-
-
-
-
-
-
6
7
1
1
-
1
-
13
-
-
1
-
-
-
5
2
19
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
6
-
7
1
1
-
1
-
-
-
1
-
-
5
2
19
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
437746
Bamforth
The molecular size of N-methyl ...
Aminobacter aminovorans
Biochem. J.
167
509-512
1977
-
-
-
-
-
-
-
-
1
-
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
437744
Bamforth
-
Solubilization and partial pur ...
Pseudomonas sp., Aminobacter aminovorans, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819
Biochem. Soc. Trans.
3
1066-1069
1975
-
-
-
-
-
-
3
7
2
-
-
3
-
13
-
-
1
-
-
-
1
1
23
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
7
2
-
-
3
-
-
-
1
-
-
1
1
23
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
437743
Hersh
N-methylglutamate dehydrogenas ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
150
219-226
1972
-
-
-
-
-
-
4
12
1
-
-
1
-
9
-
-
1
-
-
-
-
1
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
12
1
-
-
1
-
-
-
1
-
-
-
1
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437742
Hersh
An N-methyl glutamate dehydrog ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
145
115-120
1971
-
-
-
-
-
-
-
1
1
-
-
2
-
9
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-