Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21 (DE3) plysS cells | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
L-proline | in 100 mM TrisHCl, 10 mM MgCl2, 10% (v/v) glycerol, pH 8.5, at 25°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida POS-F84 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ NTA affinity column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | weak activity | Pseudomonas putida | ? | - |
? | |
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | weak activity | Pseudomonas putida POS-F84 | ? | - |
? | |
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | - |
Pseudomonas putida | DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium | - |
? | |
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | - |
Pseudomonas putida POS-F84 | DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium | - |
? | |
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | weak activity | Pseudomonas putida | ? | - |
? | |
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | weak activity | Pseudomonas putida POS-F84 | ? | - |
? | |
additional information | D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates | Pseudomonas putida | ? | - |
? | |
additional information | D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates | Pseudomonas putida POS-F84 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
L-proline:FAD oxidoreductase | - |
Pseudomonas putida |
PRODH | - |
Pseudomonas putida |
PutA | shows proline dehydrogenase activity | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | the enzyme loses a considerable amount of its original activity at above 30°C and is nearly inactivated at 70°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Pseudomonas putida | |
additional information | NAD+ and NADP+ are inert | Pseudomonas putida |