Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipids | approx. 2.5fold activation 120 min after sonication with mitochondrial phospholipids | Bos taurus |
General Stability | Organism |
---|---|
unstable, loses 30-50% activity within 48 h at 4°C, remainder of the enzyme becomes inactive after 2 weeks, complete inactivation after freezing at -20°C or -70°C overnight, enzyme is very susceptible to trypsinolysis, NADH protects from tryptic inactivation, NADPH promotes tryptic inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, aprrox. 20% inhibition, 20 mM, approx. 60% inhibition | Bos taurus | |
palmitoyl-CoA | competitive inhibition vs. NADPH | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Bos taurus | 31966 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bos taurus |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 6.3 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 6.7 | less than 50% of maximal activity above and below | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus | |
NADH | - |
Bos taurus | |
NADP+ | - |
Bos taurus | |
NADPH | - |
Bos taurus |