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Literature summary for 1.6.1.2 extracted from
- Evidence for a nicotineamide nucleotide transhydrogenase in Klebsiella pneumoniae (1994), Biochem. Biophys. Res. Commun., 198, 928-932.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| palmitoyl-CoA | - |
Escherichia coli |  |
| palmitoyl-CoA | competitive vs. NADPH | Klebsiella pneumoniae | |
| Phenylarsine oxide | 0.48 mM, 50% inhibition of acetylpyridine adenine dinucleotide reduction in inside-out membrane vesicles after 5 min | Klebsiella pneumoniae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | not clear whether this reflects a general salt effect or a Li+ specific effect | Klebsiella pneumoniae | |
| Na+ | not clear whether this reflects a general salt effect or a Na+ specific effect | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, immunoblot with antibodies against beef heart enzyme | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.26 | - |
activity in inside-out membrane vesicles at pH 7.4, presence of an uncoupler i.e. carbonylcyanide-m-chlorophylhydrazone results in 2fold stimulation | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + NADP+ | - |
Escherichia coli | NAD+ + NADPH | - |
? | |
| NADH + NADP+ | - |
Klebsiella pneumoniae | NAD+ + NADPH | - |
r | |
| NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r | |
| NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Klebsiella pneumoniae | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, immunoblot with antibodies against beef heart enzyme | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | reduction of oxidized acetylpyridine adenine dinucleotide by NADPH in inside-out membrane vesicles | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NAD+ | - |
Klebsiella pneumoniae | |
| NADH | - |
Escherichia coli | |
| NADH | - |
Klebsiella pneumoniae | |
| NADP+ | - |
Escherichia coli | |
| NADP+ | - |
Klebsiella pneumoniae | |
| NADPH | - |
Escherichia coli | |
| NADPH | - |
Klebsiella pneumoniae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00125 | - |
palmitoyl-CoA | - |
Klebsiella pneumoniae | |
| 0.007 | - |
palmitoyl-CoA | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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