Cloned (Comment) | Organism |
---|---|
all mutants expressed in soluble form in Escherichia coli BL21 | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
T66A | Km for NADH is not affected, Km for cytochrome b5 is significantly enhanced | Sus scrofa |
T66S | Km for NADH is not affected | Sus scrofa |
T66V | turnover is reduced to 10% of the native enzyme, Km for NADH is not affected, Km for cytochrome b5 is significantly enhanced | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
ferricyanide | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
0.0022 | - |
ferricyanide | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa | |
0.0025 | - |
ferricyanide | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
0.0028 | - |
NADH | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa | |
0.0028 | - |
NADH | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
0.0029 | - |
NADH | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa | |
0.0031 | - |
NADH | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
0.0031 | - |
ferricyanide | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa | |
0.013 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa | |
0.02 | - |
ferricytochrome b5 | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
0.111 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
0.125 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | Sus scrofa | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P83686 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes from Escherichia coli | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Sus scrofa | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | - |
Sus scrofa | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | Sus scrofa | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
77 | - |
ferricyanide | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
100 | - |
NADH | pH 7.0, 25°C,T66V mutant enzyme | Sus scrofa | |
314 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa | |
684 | - |
ferricyanide | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa | |
705 | - |
ferricytochrome b5 | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa | |
822 | - |
ferricyanide | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
911 | - |
ferricytochrome b5 | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
976 | - |
ferricyanide | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa | |
984 | - |
NADH | pH 7.0, 25°C,T66A mutant enzyme | Sus scrofa | |
1100 | - |
NADH | pH 7.0, 25°C, wild type enzyme | Sus scrofa | |
1150 | - |
NADH | pH 7.0, 25°C,T66S mutant enzyme | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sus scrofa | |
NADH | - |
Sus scrofa |