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Literature summary for 1.6.2.2 extracted from

  • Roma, G.W.; Crowley, L.J.; Barber, M.J.
    Expression and characterization of a functional canine variant of cytochrome b5 reductase (2006), Arch. Biochem. Biophys., 452, 69-82.
    View publication on PubMed
Search for more literature for 1.6.2.2

Protein Variants

Protein Variants Comment Organism
additional information expression of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300. Fragment retains both NADH:ferricyanide reductase and NADH:cytochrome b5 reductase activities Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 NADH pH 7.0, 25°C Canis lupus familiaris
0.008
-
 ferricyanide pH 7.0, 25°C Canis lupus familiaris
0.012
-
 ferrocytochrome b5 pH 7.0, 25°C Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31365
-
 x * 31365, mass spectrometry of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300 Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.9
-
 pH 7.0, 25°C, substrate ferricyanide Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Canis lupus familiaris 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricytochrome b5 + NADH
-
 Canis lupus familiaris 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 31365, mass spectrometry of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300 Canis lupus familiaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
600
-
 ferrocytochrome b5 pH 7.0, 25°C Canis lupus familiaris
767
-
 ferricyanide pH 7.0, 25°C Canis lupus familiaris

Cofactor

Cofactor Comment Organism Structure
NADH marked preference for NADH versus NADPH Canis lupus familiaris
NADPH marked preference for NADH versus NADPH Canis lupus familiaris