Protein Variants | Comment | Organism |
---|---|---|
G179A | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
G179P | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
G179T | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
G179V | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
ferrocytochrome b5 | mutant G179P, pH 7.0 | Rattus norvegicus | |
0.006 | - |
NADH | wild-type, pH 7.0 | Rattus norvegicus | |
0.007 | - |
ferricyanide | mutant G179P, pH 7.0 | Rattus norvegicus | |
0.007 | - |
ferricyanide | mutant G179V, pH 7.0 | Rattus norvegicus | |
0.008 | - |
ferricyanide | wild-type, pH 7.0 | Rattus norvegicus | |
0.008 | - |
ferricyanide | mutant G179A, pH 7.0 | Rattus norvegicus | |
0.008 | - |
ferrocytochrome b5 | mutant G179A, pH 7.0 | Rattus norvegicus | |
0.008 | - |
ferricyanide | mutant G179T, pH 7.0 | Rattus norvegicus | |
0.013 | - |
ferrocytochrome b5 | wild-type, pH 7.0 | Rattus norvegicus | |
0.025 | - |
NADH | mutant G179A, pH 7.0 | Rattus norvegicus | |
0.043 | - |
ferrocytochrome b5 | mutant G179T, pH 7.0 | Rattus norvegicus | |
0.107 | - |
ferrocytochrome b5 | mutant G179V, pH 7.0 | Rattus norvegicus | |
0.375 | - |
NADPH | mutant G179V, pH 7.0 | Rattus norvegicus | |
0.507 | - |
NADPH | mutant G179T, pH 7.0 | Rattus norvegicus | |
0.595 | - |
NADH | mutant G179P, pH 7.0 | Rattus norvegicus | |
0.662 | - |
NADH | mutant G179T, pH 7.0 | Rattus norvegicus | |
0.924 | - |
NADPH | wild-type, pH 7.0 | Rattus norvegicus | |
1.077 | - |
NADH | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.36 | - |
NADPH | mutant G179A, pH 7.0 | Rattus norvegicus | |
2.317 | - |
NADPH | mutant G179P, pH 7.0 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P20070 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Rattus norvegicus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricyanide + NADPH | - |
Rattus norvegicus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | - |
Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
NADPH | mutant G179V, pH 7.0 | Rattus norvegicus | |
10 | - |
ferrocytochrome b5 | mutant G179P, pH 7.0 | Rattus norvegicus | |
12 | - |
NADPH | mutant G179T, pH 7.0 | Rattus norvegicus | |
12 | - |
ferricyanide | mutant G179V, pH 7.0 | Rattus norvegicus | |
17 | - |
NADPH | mutant G179P, pH 7.0 | Rattus norvegicus | |
17 | - |
ferrocytochrome b5 | mutant G179V, pH 7.0 | Rattus norvegicus | |
18 | - |
ferrocytochrome b5 | mutant G179T, pH 7.0 | Rattus norvegicus | |
33 | - |
NADPH | wild-type, pH 7.0 | Rattus norvegicus | |
33 | - |
ferricyanide | mutant G179T, pH 7.0 | Rattus norvegicus | |
42 | - |
ferricyanide | mutant G179P, pH 7.0 | Rattus norvegicus | |
48 | - |
NADPH | mutant G179A, pH 7.0 | Rattus norvegicus | |
245 | - |
ferrocytochrome b5 | mutant G179A, pH 7.0 | Rattus norvegicus | |
400 | - |
ferrocytochrome b5 | wild-type, pH 7.0 | Rattus norvegicus | |
559 | - |
ferricyanide | mutant G179A, pH 7.0 | Rattus norvegicus | |
800 | - |
ferricyanide | wild-type, pH 7.0 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |