Literature summary for 1.6.2.4 extracted from

Fan, L.L.; Masters, B.S.S.
Properties of purified kidney microsomal NADPH-cytochrome c reductase (1974), Arch. Biochem. Biophys., 165, 665-671.

Search for more literature for 1.6.2.4

General Stability

General Stability	Organism
unstable during purification	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
NADP+	competitive inhibition	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0046	-	NADPH	-	Sus scrofa
0.01	-	ferricytochrome c	-	Sus scrofa
24	-	NADH	-	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Sus scrofa	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	68000	gel filtration, kidney	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
solubilization with lipase, column chromatography on Sephadex G-100 superfine, DEAE-cellulose and Sephadex G-200 superfine	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ferricytochrome c + NADPH	additional electron acceptor: neotetrazolium chloride	Sus scrofa	2 ferrocytochrome c + NADP+ + H+	-	?
ferricytochrome c + NADH + H+	-	Sus scrofa	ferrocytochrome c + NAD+	-	r
ferricytochrome c + NADPH + H+	-	Sus scrofa	ferrocytochrome c + NADP+	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20.5	-	ferricytochrome c	-	Sus scrofa

Cofactor

Cofactor	Comment	Organism	Structure
FAD	ratio FAD : FMN 1 : 1	Sus scrofa
FMN	ratio FAD : FMN 1 : 1	Sus scrofa

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Release 2023.1 (January 2023)