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Literature summary for 1.6.2.4 extracted from
- Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance (2008), Mol. Biochem. Parasitol., 160, 42-51.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphenyleneiodonium | 0.03 mM, 62% inhibition; 0.03 mM, 64% inhibition; 0.03 mM, 76% inhibition | Trypanosoma cruzi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00577 | - |
NADPH | pH 7.7, 30°C | Trypanosoma cruzi | |
| 0.0098 | - |
ferricytochrome c | pH 7.7, 30°C | Trypanosoma cruzi | |
| 0.017 | - |
ferricytochrome c | pH 7.7, 30°C | Trypanosoma cruzi | |
| 0.018 | - |
ferricytochrome c | pH 7.7, 30°C | Trypanosoma cruzi | |
| 0.0511 | - |
NADPH | pH 7.7, 30°C | Trypanosoma cruzi | |
| 0.05488 | - |
NADPH | pH 7.7, 30°C | Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68600 | - |
x * 68600, calculated from sequence | Trypanosoma cruzi |
| 71300 | - |
x * 71300, calculated from sequence | Trypanosoma cruzi |
| 78400 | - |
x * 78400, calculated from sequence | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q003G7 | - |
- |
| Trypanosoma cruzi | Q003G8 | - |
- |
| Trypanosoma cruzi | Q0MRD4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Trypanosoma cruzi | - |
| epimastigote | - |
Trypanosoma cruzi | - |
| epimastigote | overexpression of TcCPR-B in Trypanosoma cruzi epimastigotes increases its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole | Trypanosoma cruzi | - |
| additional information | presence in every Trypanosoma cruzi developmental stage | Trypanosoma cruzi | - |
| trypomastigote | - |
Trypanosoma cruzi | - |
| trypomastigote | strongly expressed | Trypanosoma cruzi | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | - |
Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| additional information | menadione or adrenaline are not reduced | Trypanosoma cruzi | ? | - |
? | |
| additional information | menadione or adrenaline ate not reduced | Trypanosoma cruzi | ? | - |
? | |
| NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Trypanosoma cruzi | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68600, calculated from sequence | Trypanosoma cruzi |
| ? | x * 71300, calculated from sequence | Trypanosoma cruzi |
| ? | x * 78400, calculated from sequence | Trypanosoma cruzi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPR | - |
Trypanosoma cruzi |
| NADPH-cytochrome P450 reductase | - |
Trypanosoma cruzi |
| TcCPR-A | - |
Trypanosoma cruzi |
| TcCPR-B | - |
Trypanosoma cruzi |
| TcCPR-C | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Trypanosoma cruzi | |
| ferricytochrome c | - |
Trypanosoma cruzi | |
| FMN | - |
Trypanosoma cruzi | |
| NADPH | - |
Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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