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Literature summary for 1.6.2.4 extracted from

  • Rana, S.; Lattoo, S.K.; Dhar, N.; Razdan, S.; Bhat, W.W.; Dhar, R.S.; Vishwakarma, R.
    NADPH-cytochrome P450 reductase: molecular cloning and functional characterization of two paralogs from Withania somnifera (L.) dunal (2013), PLoS ONE, 8, e57068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
WsCPR1, phylogenetic analysis and quantitative real-time PCR expression analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Withania somnifera
WsCPR2, phylogenetic analysis and quantitative real-time PCR expression analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Withania somnifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Withania somnifera
0.00506
-
 NADPH purified recombinant enzyme, pH 7.8, 25°C Withania somnifera
0.00648
-
 NADPH purified recombinant enzyme, pH 7.8, 25°C Withania somnifera

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme contains membrane anchor regions Withania somnifera 16020
-
microsome
-
 Withania somnifera
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + H+ + cytochrome c Withania somnifera
-
 NADP+ + reduced cytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Withania somnifera D6P3J1 WsCPR2; WsCPR2
-
Withania somnifera I1E3I6 WsCPR1; WsCPR1
-

Purification (Commentary)

Purification (Comment) Organism
WsCPR1, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, cleavage of the tag by thrombin, and elimination of thrombin by benzymedene beads Withania somnifera
WsCPR2, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, cleavage of the tag by thrombin, and elimination of thrombin by benzymedene beads Withania somnifera

Source Tissue

Source Tissue Comment Organism Textmining
berry
-
 Withania somnifera
-
flower
-
 Withania somnifera
-
leaf
-
 Withania somnifera
-
additional information higher expression level of WsCPR2 in comparison to WsCPR1 Withania somnifera
-
root
-
 Withania somnifera
-
stem
-
 Withania somnifera
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.83
-
 purified recombinant enzyme, pH 7.8, 25°C Withania somnifera
7.56
-
 purified recombinant enzyme, pH 7.8, 25°C Withania somnifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + cytochrome c
-
 Withania somnifera NADP+ + reduced cytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
NADPH-cytochrome P450 reductase
-
 Withania somnifera
WsCPR1
-
 Withania somnifera
WsCPR2
-
 Withania somnifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Withania somnifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Withania somnifera

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Withania somnifera

Expression

Organism Comment Expression
Withania somnifera WsCPR1 is uninducible while WsCPR2 transcript level increase in a time-dependent manner after elicitor treatments, e.g. by methyljasmonate or salicylic acid up

General Information

General Information Comment Organism
evolution grouping of dual Cytochrome P450 reductases, CPRs, is in accordance with class I and class II of eudicotyledon CPRs, overview Withania somnifera
metabolism involvement of the enzyme in the withanolide biosynthesic pathway, overview Withania somnifera
physiological function cytochrome P450 reductase is the most imperative redox partner of multiple P450s involved in primary and secondary metabolite biosynthesis Withania somnifera