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Literature summary for 1.6.2.4 extracted from
- A novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase (2016), J. Biotechnol., 231, 83-94 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C43(DE3) cells | Priestia megaterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0167 | - |
ferricytochrome c | at pH 7.5 and 25°C | Priestia megaterium |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
| Priestia megaterium DSM319 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + H+ + ferricytochrome c | - |
Priestia megaterium | NADP+ + ferrocytochrome c | - |
? | |
| NADPH + H+ + ferricytochrome c | - |
Priestia megaterium DSM319 | NADP+ + ferrocytochrome c | - |
? | |
| NADPH + H+ + oxidized CYP106A1 | - |
Priestia megaterium | NADP+ + reduced CYP106A1 | - |
? | |
| NADPH + H+ + oxidized CYP106A1 | - |
Priestia megaterium DSM319 | NADP+ + reduced CYP106A1 | - |
? | |
| NADPH + H+ + oxidized CYP21A2 | - |
Priestia megaterium | NADP+ + reduced CYP21A2 | - |
? | |
| NADPH + H+ + oxidized CYP21A2 | - |
Priestia megaterium DSM319 | NADP+ + reduced CYP21A2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPR | - |
Priestia megaterium |
| cytochrome P450 reductase | - |
Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 54 | - |
the reductase loses 50% of its activity after 10 min at 54°C | Priestia megaterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43.03 | - |
ferricytochrome c | at pH 7.5 and 25°C | Priestia megaterium | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Priestia megaterium | |
| FMN | - |
Priestia megaterium | |
| NADPH | - |
Priestia megaterium | |
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Release 2023.1 (January 2023)
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