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Literature summary for 1.6.2.5 extracted from
- Isolation, purification, and some properties of reduced nicotinamide adenine dinucleotide phosphate-cytochrome C2 reductase from Rhodopseudomonas spheroides (1968), J. Biol. Chem., 243, 3742-3749.
General Stability
| General Stability | Organism |
|---|---|
| activity is lost overnight if the enzyme is diluted | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Cereibacter sphaeroides |  |
| N-ethylmaleimide | - |
Cereibacter sphaeroides | |
| NADH | competitive inhibitor | Cereibacter sphaeroides | |
| p-chloromercuribenzoate | - |
Cereibacter sphaeroides | |
| thyroxine | - |
Cereibacter sphaeroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0125 | - |
2,6-dichloroindophenol | - |
Cereibacter sphaeroides | |
| 0.037 | - |
ferricytochrome c2 | - |
Cereibacter sphaeroides | |
| 0.125 | - |
ferricytochrome c2 | - |
Cereibacter sphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c2 + NADPH | Rhodopseudomonas palustris | - |
2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c2 + NADPH | Cereibacter sphaeroides | native cytochrome is the natural physiological electron acceptor | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
| Rhodopseudomonas palustris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cereibacter sphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 164 | - |
- |
Cereibacter sphaeroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, can be stored as lyophilized powder for at least 3 months | Cereibacter sphaeroides |
| 4°C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c2 + NADPH | - |
Rhodopseudomonas palustris | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c2 + NADPH | contains additional NADP+ diaphorase activity | Cereibacter sphaeroides | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c2 + NADPH | contains additional NADP+ diaphorase activity | Rhodopseudomonas palustris | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c2 + NADPH | native cytochrome is the natural physiological electron acceptor | Cereibacter sphaeroides | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
| 2,6-dichloroindophenol + NADPH | - |
Cereibacter sphaeroides | ? + NADP+ | - |
? | |
| Fe(CN)6 3- + NADPH | - |
Cereibacter sphaeroides | Fe(CN)6 4- + NADP+ | - |
? | |
| horse heart cytochrome c + NADPH | - |
Cereibacter sphaeroides | ? + NADP+ | - |
? | |
| mammalian cytochrome c + NADPH | - |
Cereibacter sphaeroides | ? + NADP+ | - |
? | |
| additional information | cytochrome c-553 is ineffective as an electron acceptor | Cereibacter sphaeroides | ? | - |
? | |
| NADPH + H+ + ferricytochrome c2 | - |
Cereibacter sphaeroides | NADP+ + ferrocytochrome c2 | - |
r | |
| Rhodospirillum rubrum cytochrome c2 + NADPH | - |
Cereibacter sphaeroides | ? + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-cytochrome f reductase | - |
Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8 | - |
Cereibacter sphaeroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | nonmetalloflavoprotein with FAD as prosthetic group | Cereibacter sphaeroides | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
NADH | - |
Cereibacter sphaeroides | |
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