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Literature summary for 1.6.2.6 extracted from

  • Becana, M.; Klucas, R.V.
    Enzymatic and nonenzymatic mechanisms for ferric leghemoglobin reduction in legume root nodules (1990), Proc. Natl. Acad. Sci. USA, 87, 7295-7299.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Amobarbital 1 mM, 35% after 0.5-1 h, 80% after 4 h Glycine max
catalase possibly H2O2 as reaction intermediate Glycine max
iodoacetamide 1 mM, 95-100% inhibition Glycine max
nicotinate ferrileghemoglobin-nitrite complex Glycine max
p-hydroxymercuribenzoate 0.5 mM, slight inhibition Glycine max
Quinacrine 0.5 mM, 30-40% inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0133
-
ferrileghemoglobin
-
Phaseolus vulgaris
0.0133
-
ferrileghemoglobin
-
Vigna unguiculata
0.088
-
ferrileghemoglobin
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, SDS-PAGE Glycine max
55000
-
2 * 55000, SDS-PAGE Vigna unguiculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD(P)H + ferrileghemoglobin Glycine max
-
NAD(P)+ + ferroleghemoglobin
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-
Phaseolus vulgaris
-
bean
-
Vigna unguiculata
-
cowpea
-

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
Phaseolus vulgaris
-
root nodule
-
Glycine max
-
root nodule
-
Vigna unguiculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.215
-
reduction of ferrileghemoglobin Glycine max
2
-
reduction of 2,6-dichlorophenolindophenol Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + ferrileghemoglobin
-
Glycine max NAD(P)+ + ferroleghemoglobin
-
?
NAD(P)H + ferrileghemoglobin
-
Vigna unguiculata NAD(P)+ + ferroleghemoglobin
-
?
NADH + H+ + ferrileghemoglobin
-
Phaseolus vulgaris NAD+ + ferroleghemoglobin
-
r

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Glycine max
dimer 2 * 55000, SDS-PAGE Vigna unguiculata

Cofactor

Cofactor Comment Organism Structure
NADH
-
Phaseolus vulgaris
NADH
-
Glycine max
NADH
-
Vigna unguiculata
NADPH 54-80% of NADH activity Phaseolus vulgaris
NADPH 54-80% of NADH activity Glycine max
NADPH 54-80% of NADH activity Vigna unguiculata