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Literature summary for 1.6.3.4 extracted from
- Characteristics of a water-forming NADH oxidase from Methanobrevibacter smithii, an archaeon in the human gut (2016), Biosci. Rep., 36, e00410 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Methanobrevibacter smithii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + O2 | Methanobrevibacter smithii | - |
NAD+ + H2O | - |
? |  |
| NADH + H+ + O2 | Methanobrevibacter smithii ATCC 35061 | - |
NAD+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobrevibacter smithii | - |
- |
- |
| Methanobrevibacter smithii ATCC 35061 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Methanobrevibacter smithii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + O2 | - |
Methanobrevibacter smithii | NAD+ + H2O | - |
? | |
| NADH + H+ + O2 | - |
Methanobrevibacter smithii ATCC 35061 | NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Methanobrevibacter smithii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NOX | - |
Methanobrevibacter smithii |
| water-forming NADH oxidase | - |
Methanobrevibacter smithii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Methanobrevibacter smithii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Methanobrevibacter smithii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | the enzyme is most active between pH values 6.5-8.0 | Methanobrevibacter smithii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Methanobrevibacter smithii | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanobrevibacter smithii | calculated from amino acid sequence | - |
4.8 |
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