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Literature summary for 1.6.3.4 extracted from

  • Zhang, X.; Zhang, R.; Bao, T.; Rao, Z.; Yang, T.; Xu, M.; Xu, Z.; Li, H.; Yang, S.
    The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis (2014), Metab. Eng., 23, 34-41 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in bdhA gene-disrupted Bacillus subtilis Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 NADH at pH 9.0 and 35°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis no activity with NADPH ?
-
 ?
additional information Bacillus subtilis 168 no activity with NADPH ?
-
 ?
NADH + H+ + O2 Bacillus subtilis
-
 NAD+ + H2O
-
 ?
NADH + H+ + O2 Bacillus subtilis 168
-
 NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NADPH Bacillus subtilis ?
-
 ?
additional information no activity with NADPH Bacillus subtilis 168 ?
-
 ?
NADH + H+ + O2
-
 Bacillus subtilis NAD+ + H2O
-
 ?
NADH + H+ + O2
-
 Bacillus subtilis 168 NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
NOX
-
 Bacillus subtilis
water-forming NADH oxidase
-
 Bacillus subtilis
YODC
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 after incubation between pH 6.0 and 8.0 for 2 h, the enzyme maintains more than 90% of the whole activity Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Bacillus subtilis