Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
homology modeling and substrate docking, the nicotinamide ring of NADH extends vertically toward to re-face of coenzyme (FAD) | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 1% residual activity | Streptococcus mutans | |
Li+ | 1 mM, 80% residual activity | Streptococcus mutans | |
Mg2+ | 1 mM, 85% residual activity | Streptococcus mutans | |
Ni2+ | 1 mM, 72% residual activity | Streptococcus mutans | |
Triton X-100 | - |
Streptococcus mutans | |
Tween-20 | - |
Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0577 | - |
NADH | pH 7, 25°C | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Streptococcus mutans ATCC 25175 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
281.2 | - |
pH 7, 35°C | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 NADH + 2 H+ + O2 | - |
Streptococcus mutans | 2 NAD+ + 2 H2O | - |
? | |
2 NADH + 2 H+ + O2 | - |
Streptococcus mutans ATCC 25175 | 2 NAD+ + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49900, SDS-PAGE, x * 49930, calculated from sequence | Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptococcus mutans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 25 | 72-79% of maximum activity | Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
3 h, almost 100% residual activity | Streptococcus mutans |
35 | - |
3 h, 71% residual activity | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | optimum concentration 0.3 mM | Streptococcus mutans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus mutans | calculated from sequence | - |
5.4 |