Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-Naphthoquinone | substrate inhibition at concentrations above 0.025 mM | Pseudomonas aeruginosa | |
coenzyme Q0 | substrate inhibition at concentrations above 0.025 mM | Pseudomonas aeruginosa | |
tetramethyl-1,4-benzoquinone | substrate inhibition at concentrations above 0.025 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
NADPH | pH 6, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I4B3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADPH + H+ | - |
Pseudomonas aeruginosa | 1,4-benzoquinol + NADP+ | - |
? | |
2,6-dimethoxy-1,4-benzoquinone + NADPH + H+ | - |
Pseudomonas aeruginosa | 2,6-dimethoxy-1,4-benzoquinol + NADP+ | - |
? | |
additional information | PA1225 does not react with methyl red and does not possess azoreductase activity and displays a negligible NADPH:O2 oxidase activity | Pseudomonas aeruginosa | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PA1225 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
2,6-dimethoxy-1,4-benzoquinone | pH 6, 25°C | Pseudomonas aeruginosa | |
5.9 | - |
1,4-benzoquinone | pH 6, 25°C | Pseudomonas aeruginosa | |
10.1 | - |
NADPH | pH 6, 25°C | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme utilizes FAD to transfer a hydride ion from NADPH to quinones | Pseudomonas aeruginosa | |
NADH | efficiency with NADH is 40fold lower than with NADPH | Pseudomonas aeruginosa | |
NADPH | efficiency with NADH is 40fold lower than with NADPH | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | lack of inhibition by NADP+ versus NADPH at saturating concentrations of 1,4-benzoquinone is consistent with a ping-pong bi-bi mechanism. The reductive half-reaction at pH 6.0 has Kd values of 0.07 mM with NADPH and 1.8 mM with NADH. The kred for flavin reduction is independent of pH with values of about 10 per s with NADPH and about 5 per s with NADH. Hydride transfer from NADPH to FAD is fully rate limiting for the overall turnover of the enzyme | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
124 | - |
NADPH | pH 6, 25°C | Pseudomonas aeruginosa |