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Literature summary for 1.6.5.10 extracted from

  • Flores, E.; Gadda, G.
    Kinetic characterization of PA1225 from Pseudomonas aeruginosa PAO1 reveals a new NADPH quinone reductase (2018), Biochemistry, 57, 3050-3058 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,4-Naphthoquinone substrate inhibition at concentrations above 0.025 mM Pseudomonas aeruginosa
coenzyme Q0 substrate inhibition at concentrations above 0.025 mM Pseudomonas aeruginosa
tetramethyl-1,4-benzoquinone substrate inhibition at concentrations above 0.025 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
NADPH pH 6, 25°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4B3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NADPH + H+
-
Pseudomonas aeruginosa 1,4-benzoquinol + NADP+
-
?
2,6-dimethoxy-1,4-benzoquinone + NADPH + H+
-
Pseudomonas aeruginosa 2,6-dimethoxy-1,4-benzoquinol + NADP+
-
?
additional information PA1225 does not react with methyl red and does not possess azoreductase activity and displays a negligible NADPH:O2 oxidase activity Pseudomonas aeruginosa ?
-
-

Synonyms

Synonyms Comment Organism
PA1225
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
2,6-dimethoxy-1,4-benzoquinone pH 6, 25°C Pseudomonas aeruginosa
5.9
-
1,4-benzoquinone pH 6, 25°C Pseudomonas aeruginosa
10.1
-
NADPH pH 6, 25°C Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD enzyme utilizes FAD to transfer a hydride ion from NADPH to quinones Pseudomonas aeruginosa
NADH efficiency with NADH is 40fold lower than with NADPH Pseudomonas aeruginosa
NADPH efficiency with NADH is 40fold lower than with NADPH Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism lack of inhibition by NADP+ versus NADPH at saturating concentrations of 1,4-benzoquinone is consistent with a ping-pong bi-bi mechanism. The reductive half-reaction at pH 6.0 has Kd values of 0.07 mM with NADPH and 1.8 mM with NADH. The kred for flavin reduction is independent of pH with values of about 10 per s with NADPH and about 5 per s with NADH. Hydride transfer from NADPH to FAD is fully rate limiting for the overall turnover of the enzyme Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
124
-
NADPH pH 6, 25°C Pseudomonas aeruginosa