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Literature summary for 1.6.5.2 extracted from
- Purification and Ccaracterization of a 1,4-benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium (1995), Appl. Environ. Microbiol., 61, 3076-3081 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cibacron blue 3GA | - |
Phanerodontia chrysosporium |  |
| dicumarol | - |
Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
2-methoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.0074 | - |
2,6-dimethoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.0105 | - |
2,6-dichlorophenolindophenol | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.0208 | - |
2,5-di-tert-butyl-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.032 | - |
menadione | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.048 | - |
NADPH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 0.055 | - |
NADH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
gel filtration | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q9Y763 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 217 | - |
pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-di-tert-butyl-1,4-benzoquinone + NADH | - |
Phanerodontia chrysosporium | 2,5-di-tert-butyl-1,4-benzoquinol + NAD+ | - |
? | |
| 2,6-dichlorophenolindophenol + NADPH | - |
Phanerodontia chrysosporium | reduced 2,6-dichlorophenolindophenol + NADP+ | - |
? | |
| 2,6-dimethoxy-1,4-benzoquinone + NADH | - |
Phanerodontia chrysosporium | 2,6-dimethoxy-1,4-benzoquinol + NAD+ | - |
? | |
| 2-methoxy-1,4-benzoquinone + NADPH | - |
Phanerodontia chrysosporium | 2-methoxy-1,4-benzoquinol + NADP+ | - |
? | |
| menadione + NADH | - |
Phanerodontia chrysosporium | reduced menadione + NAD+ | - |
? | |
| additional information | catalyzes the two-electron reduction of several quinones and other electron acceptors utilizing either NADH or NADPH as an electron donor | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,4-benzoquinone reductase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 330 | - |
2,6-dichlorophenolindophenol | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 560 | - |
2,6-dimethoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 760 | - |
NADPH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 800 | - |
NADH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 1400 | - |
2,5-di-tert-butyl-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 4900 | - |
menadione | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 440000 | - |
2-methoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | - |
Phanerodontia chrysosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | absoprtion maxima at 375 and 450 nm | Phanerodontia chrysosporium | |
| NADH | - |
Phanerodontia chrysosporium | |
| NADPH | - |
Phanerodontia chrysosporium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phanerodontia chrysosporium | isoelectric focusing | - |
4.3 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Phanerodontia chrysosporium | expressed in cultures grown under both nitrogen-sufficient and nitrogen-limiting conditions | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
NADH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 16 | - |
NADPH | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 31 | - |
2,6-dichlorophenolindophenol | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 67 | - |
2,5-di-tert-butyl-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 150 | - |
menadione | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 760 | - |
2,6-dimethoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
| 180000 | - |
2-methoxy-1,4-benzoquinone | pH 6.0, temperature not specified in the publication | Phanerodontia chrysosporium | |
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