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Literature summary for 1.6.5.4 extracted from
- Monodehydroascorbate reductase from cucumber is a flavin adenine dinucleotide enzyme (1985), J. Biol. Chem., 260, 12920-12926.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Iodoacetamide | 25 mM, 21% inhibition | Cucumis sativus |  |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cucumis sativus | |
| NEM | 25 mM, 34% inhibition | Cucumis sativus | |
| PCMB | 0.05 mM, 82% inhibition | Cucumis sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
NADH | - |
Cucumis sativus | |
| 0.012 | - |
ferricyanide | - |
Cucumis sativus | |
| 0.023 | - |
NADPH | - |
Cucumis sativus | |
| 0.03 | - |
2,6-dichloroindophenol | - |
Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucumis sativus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate | ping pong mechanism | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 256 | - |
- |
Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferricyanide + NADPH + H+ | - |
Cucumis sativus | ferrocyanide + NADP+ | - |
r | |
| NADH + 2,6-dichlorophenol indophenol | - |
Cucumis sativus | NAD+ + ? | - |
? | |
| NADH + monodehydroascorbate | - |
Cucumis sativus | NAD+ + ascorbate | - |
? | |
| NADPH + monodehydroascorbate | - |
Cucumis sativus | NADP+ + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Cucumis sativus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
NADPH | - |
Cucumis sativus | |
| 200 | - |
NADH | - |
Cucumis sativus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Cucumis sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | pH 5.5: about 60% of maximal activity, pH 9.5: about 75% of maximal activity | Cucumis sativus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Cucumis sativus | |
| NADPH | - |
Cucumis sativus | |
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Release 2023.1 (January 2023)
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