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Literature summary for 1.6.5.4 extracted from

  • Li, F.; Wu, Q.; Duan, M.; Dong, X.; Li, B.; Meng, Q.
    Transgenic tomato plants overexpressing chloroplastic monodehydroascorbate reductase are resistant to salt- and PEG-induced osmotic stress (2012), Photosynthetica, 50, 120-128.
No PubMed abstract available
Search for more literature for 1.6.5.4

Protein Variants

Protein Variants Comment Organism
additional information overexpression of MDHAR enhanced tolerance to osmotic stress Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Solanum lycopersicum 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + 2 monodehydroascorbate Solanum lycopersicum
-
 NAD+ + 2 ascorbate
-
 ?
NADH + H+ + 2 monodehydroascorbate Solanum lycopersicum Zhongshu 4
-
 NAD+ + 2 ascorbate
-
 ?
NADPH + H+ + 2 monodehydroascorbate Solanum lycopersicum
-
 NADP+ + 2 ascorbate
-
 ?
NADPH + H+ + 2 monodehydroascorbate Solanum lycopersicum Zhongshu 4
-
 NADP+ + 2 ascorbate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-
Solanum lycopersicum Zhongshu 4
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf higher enzyme expression level in leaves compared to stems Solanum lycopersicum
-
stem
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum NAD+ + 2 ascorbate
-
 ?
NADH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum Zhongshu 4 NAD+ + 2 ascorbate
-
 ?
NADPH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum NADP+ + 2 ascorbate
-
 ?
NADPH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum Zhongshu 4 NADP+ + 2 ascorbate
-
 ?

Synonyms

Synonyms Comment Organism
MDHAR
-
 Solanum lycopersicum
monodehydroascorbate reductase
-
 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
additional information MDHAR catalyses the reduction of the MDHA to AsA, using NADH or NADPH as an electron donor Solanum lycopersicum
NADH
-
 Solanum lycopersicum
NADPH
-
 Solanum lycopersicum

Expression

Organism Comment Expression
Solanum lycopersicum the MDHAR expression level is decreased firstly and then increased, the maximum level is observed after treatment for 12 h with 200 mM NaCl or 20% PEG6000, overview. The stressful treatment accelerates degradation of mRNA at the early stage, then with the duration of treatment the plants respond to the stress and the synthesis of MDHAR increases to protect the plant from stressful damage up

General Information

General Information Comment Organism
additional information overexpression of MDHAR enhanced tolerance to osmotic stress Solanum lycopersicum
physiological function the enzyme is involved in maintaining the reactive oxygen species scavenging capability of plant cells, as regeneration of ascorbate from its primary oxidation product monodehydroascorbate, an essential process and obviously indispensable. With its ability to directly regenerate ascorbate, MDHAR probably plays an important role in the plant antioxidant system by maintaining the pool of ascorbate. Chloroplastic MDHAR plays an important role in alleviating photoinhibition of PSII by elevating ascorbate (AsA) level under salt- and PEG-induced osmotic stress Solanum lycopersicum