BRENDA - Enzyme Database show
show all sequences of 1.6.5.5

Identification and characterization of the enzymatic activity of zeta-Crystallin from guinea pig lens

Rao, P.V.; Krishna, C.M.; Zigler, J.S.; J. Biol. Chem. 267, 96-102 (1992)

Data extracted from this reference:

General Stability
General Stability
Organism
NADPH protects against inactivation caused by heat, NEM or H2O2
Cavia porcellus
very stable to freezing
Cavia porcellus
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Cavia porcellus
Cibacron blue 3GA
-
Cavia porcellus
dicoumarol
-
Cavia porcellus
H2O2
-
Cavia porcellus
additional information
no inactivation by 0.5 mM iodoacetate
Cavia porcellus
NADP+
-
Cavia porcellus
NEM
-
Cavia porcellus
Nitrofurantoin
-
Cavia porcellus
p-Chloromercuriphenylsulfonate
-
Cavia porcellus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
-
Cavia porcellus
0.01
-
9,10-phenanthrenequinone
-
Cavia porcellus
0.027
-
5-hydroxy-1,4-naphthoquinone
-
Cavia porcellus
0.143
-
1,4-benzoquinone
-
Cavia porcellus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cavia porcellus
-
-
-
Reaction
Reaction
Commentary
Organism
NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone
enzyme acts through a one-electron transfer process
Cavia porcellus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
lens
-
Cavia porcellus
-
Storage Stability
Storage Stability
Organism
-20°C, less than 10% loss of activity after 4-6 weeks
Cavia porcellus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-naphthoquinone + NADPH
-
14077
Cavia porcellus
1,2-naphthoquinol + NADP+
-
-
-
-
1,4-benzoquinone + NADPH
15.3% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
1,4-benzoquinol + NADP+
-
-
-
-
1,4-naphthoquinone + NADPH
2.6% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
1,4-naphthoquinol + NADP+
-
-
-
-
5-hydroxy-1,4-naphthoquinone + NADPH
i.e. juglone, 12.5% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
5-hydroxy-1,4-naphthoquinol + NADP+
-
-
-
-
5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH
i.e. plumbagin, 0.9% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+
-
-
-
-
9,10-phenanthrenequinone + NADPH
50% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
9,10-phenanthrenequinol + NADP+
-
-
-
-
methyl-1,4-benzoquinone + NADPH
20.6% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
methyl-1,4-benzoquinol + NADP+
-
-
-
-
additional information
inactive with: menadione, ubiquinone, 9,10-anthraquinone, vitamin K1, vitamin K2
14077
Cavia porcellus
?
-
-
-
-
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
3.8% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, 65% loss of activity
Cavia porcellus
51
-
10 min, complete loss of activity
Cavia porcellus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.6
-
5-hydroxy-1,4-naphthoquinone
-
Cavia porcellus
3.4
-
1,4-benzoquinone
-
Cavia porcellus
10
-
9,10-phenanthrenequinone
-
Cavia porcellus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
specific for
Cavia porcellus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
specific for
Cavia porcellus
General Stability (protein specific)
General Stability
Organism
NADPH protects against inactivation caused by heat, NEM or H2O2
Cavia porcellus
very stable to freezing
Cavia porcellus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Cavia porcellus
Cibacron blue 3GA
-
Cavia porcellus
dicoumarol
-
Cavia porcellus
H2O2
-
Cavia porcellus
additional information
no inactivation by 0.5 mM iodoacetate
Cavia porcellus
NADP+
-
Cavia porcellus
NEM
-
Cavia porcellus
Nitrofurantoin
-
Cavia porcellus
p-Chloromercuriphenylsulfonate
-
Cavia porcellus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
-
Cavia porcellus
0.01
-
9,10-phenanthrenequinone
-
Cavia porcellus
0.027
-
5-hydroxy-1,4-naphthoquinone
-
Cavia porcellus
0.143
-
1,4-benzoquinone
-
Cavia porcellus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
lens
-
Cavia porcellus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, less than 10% loss of activity after 4-6 weeks
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-naphthoquinone + NADPH
-
14077
Cavia porcellus
1,2-naphthoquinol + NADP+
-
-
-
-
1,4-benzoquinone + NADPH
15.3% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
1,4-benzoquinol + NADP+
-
-
-
-
1,4-naphthoquinone + NADPH
2.6% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
1,4-naphthoquinol + NADP+
-
-
-
-
5-hydroxy-1,4-naphthoquinone + NADPH
i.e. juglone, 12.5% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
5-hydroxy-1,4-naphthoquinol + NADP+
-
-
-
-
5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH
i.e. plumbagin, 0.9% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+
-
-
-
-
9,10-phenanthrenequinone + NADPH
50% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
9,10-phenanthrenequinol + NADP+
-
-
-
-
methyl-1,4-benzoquinone + NADPH
20.6% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
methyl-1,4-benzoquinol + NADP+
-
-
-
-
additional information
inactive with: menadione, ubiquinone, 9,10-anthraquinone, vitamin K1, vitamin K2
14077
Cavia porcellus
?
-
-
-
-
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
3.8% of the activity with 1,2-naphthoquinone
14077
Cavia porcellus
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, 65% loss of activity
Cavia porcellus
51
-
10 min, complete loss of activity
Cavia porcellus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.6
-
5-hydroxy-1,4-naphthoquinone
-
Cavia porcellus
3.4
-
1,4-benzoquinone
-
Cavia porcellus
10
-
9,10-phenanthrenequinone
-
Cavia porcellus
Other publictions for EC 1.6.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741786
Valiauga
Reduction of quinones and nit ...
Escherichia coli
Arch. Biochem. Biophys.
614
14-22
2017
-
2
-
-
-
-
1
17
-
-
-
-
-
2
-
-
-
1
-
-
-
-
17
-
1
-
-
16
1
-
-
2
-
-
-
-
2
-
2
-
-
-
-
1
-
17
-
-
-
-
-
-
-
-
-
-
-
-
17
-
1
-
-
16
1
-
-
-
-
2
2
-
16
16
742492
Crosas
The yeast zeta-crystallin/NAD ...
Saccharomyces cerevisiae
FEBS J.
282
1953-1964
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
742325
Alharbi
-
Inhibition of NADPH quinone o ...
Camelus sp.
Curr. Enzyme Inhib.
10
137-142
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
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-
-
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1
-
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-
-
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1
1
-
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-
-
-
-
-
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724333
Hervas
ArsH from the cyanobacterium S ...
Synechocystis sp.
Biochemistry
51
1178-1187
2012
-
-
1
-
-
-
2
9
-
-
2
1
-
3
-
-
-
1
-
-
-
-
10
1
1
-
-
7
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
9
-
-
2
1
-
-
-
-
-
-
-
-
10
1
1
-
-
7
1
-
-
-
-
2
2
-
-
-
724703
Porte
Kinetic and structural evidenc ...
Homo sapiens
Cell. Mol. Life Sci.
68
1065-1077
2011
-
-
1
1
2
-
-
22
-
-
-
-
-
1
-
-
1
-
-
-
-
-
9
2
-
-
-
19
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
22
-
-
-
-
-
-
-
1
-
-
-
-
9
2
-
-
-
19
-
-
-
-
-
-
-
-
19
19
701074
Simpanya
Expression and purification of ...
Mus musculus
Protein Expr. Purif.
69
147-152
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
696058
Pan
Crystal structures of Pseudomo ...
Pseudomonas syringae
Biochem. Biophys. Res. Commun.
390
597-602
2009
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
697278
Porte
MDR quinone oxidoreductases: t ...
Homo sapiens, Saccharomyces cerevisiae
Chem. Biol. Interact.
178
288-294
2009
-
-
-
-
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
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-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697208
Fernandez
Human and yeast zeta-crystalli ...
Homo sapiens, Saccharomyces cerevisiae
Cell. Mol. Life Sci.
64
1419-1427
2007
-
-
2
-
-
-
-
6
-
-
4
2
-
4
-
-
2
-
-
2
-
2
8
2
-
-
-
4
2
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
6
-
-
4
2
-
-
-
2
-
2
-
2
8
2
-
-
-
4
2
-
-
-
-
-
-
-
-
-
671365
Uchiyama
Antichagasic activity of komar ...
Trypanosoma cruzi
Antimicrob. Agents Chemother.
49
5123-5126
2005
-
-
-
-
-
-
-
3
1
-
-
-
-
2
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
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1
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-
-
1
-
-
-
-
-
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3
1
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676693
Greenshields
Differential regulation of whe ...
Triticum monococcum
Planta
222
867-875
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
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-
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1
-
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-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655855
Shimomura
Crystal structures of the quin ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
J. Bacteriol.
185
4211-4218
2003
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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1
-
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-
1
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-
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1
1
-
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-
1
-
-
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-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
716132
Maruyama
Oxidative-stress-inducible qor ...
Staphylococcus aureus
Microbiology
149
389-398
2003
-
-
1
-
-
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
2
-
1
-
-
-
1
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
697701
Mano
A novel NADPH:diamide oxidored ...
Arabidopsis thaliana, Cavia porcellus
Eur. J. Biochem.
267
3661-3671
2000
-
-
1
-
-
-
4
9
1
-
2
-
-
7
-
-
2
-
-
1
-
-
19
1
-
-
-
16
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
4
-
9
1
-
2
-
-
-
-
2
-
1
-
-
19
1
-
-
-
16
1
1
-
-
-
-
-
-
-
-
14086
Rabbani
Inhibition of camel lens zeta- ...
Camelus dromedarius
Biochim. Biophys. Acta
1388
175-180
1998
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14084
Abdulaziz
Inhibition of camel lens zeta- ...
Camelus dromedarius
Biochem. Mol. Biol. Int.
41
415-421
1997
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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14089
Rao
Guinea pig and bovine zeta-cry ...
Bos taurus, Cavia porcellus
Biochemistry
36
5353-5362
1997
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2
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6
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14079
Duhaiman
Involvement of a disulfide bri ...
Camelus dromedarius
Biochem. Biophys. Res. Commun.
221
229-233
1996
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3
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1
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-
-
14082
Duhaiman
Inhibition of camel lens zeta- ...
Camelus dromedarius
J. Enzyme Inhib.
10
263-269
1996
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1
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2
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2
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1
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14083
Duhaiman
Camel lens zeta-crystallin kin ...
Camelus dromedarius
Biochem. Mol. Biol. Int.
38
251-258
1996
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1
2
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2
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1
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-
14087
Duhaiman
Inhibition of zeta-crystallin ...
Camelus dromedarius
J. Protein Chem.
15
261-264
1996
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4
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2
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2
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2
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-
-
-
-
14088
Duhaiman
Kinetic properties of camel le ...
Camelus dromedarius
Int. J. Biochem. Cell Biol.
28
1163-1168
1996
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-
13
6
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2
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1
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5
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13
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6
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2
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5
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-
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-
-
14081
Duhaiman
Purification and characterizat ...
Camelus dromedarius
Biochem. Biophys. Res. Commun.
215
632-640
1995
-
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1
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2
-
-
2
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2
-
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3
1
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6
1
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1
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1
1
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2
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3
1
2
6
1
1
1
-
-
1
1
-
-
-
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-
-
-
-
14077
Rao
Identification and characteriz ...
Cavia porcellus
J. Biol. Chem.
267
96-102
1992
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-
-
2
9
4
-
-
-
-
-
4
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1
-
3
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9
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2
3
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1
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9
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4
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3
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1
9
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2
3
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-
14085
Rao
Purification and characterizat ...
Cavia porcellus, Homo sapiens
Biochim. Biophys. Acta
1117
315-320
1992
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-
-
-
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-
2
2
-
4
-
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1
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1
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2
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3
1
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6
1
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-
-
-
-
-
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-
-
-
715414
Nutter
DNA strand scission and free r ...
Homo sapiens
J. Biol. Chem.
267
2474-2479
1992
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
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1
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1
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-
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-
-
-
-
-
-
-
-
-
697795
Huang
Association of hereditary cata ...
Cavia porcellus 13/N, Cavia porcellus
Exp. Eye Res.
50
317-325
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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