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Literature summary for 1.6.5.7 extracted from

  • Zaborina, O.; Daubaras, D.L.; Zago, A.; Xun, L.; Saido, K; Klem, T.; Nikolic, D.; Chakrabarty, A.M.
    Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100 (1998), J. Bacteriol., 180, 4667-4675.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
2 * 22000, SDS-PAGE Burkholderia cepacia
50000
-
gel filtration, Sephacryl S100 Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia AC1100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3-step purification, 27fold enrichment Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
with succinate as carbon source Burkholderia cepacia
68
-
after induction with 2,4,5-trichlorophenoxyacetic acid Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-[1,4]-benzoquinone + NADH
-
Burkholderia cepacia 1,2,4-trihydroxybenzene + NAD+
-
r
2-hydroxy-[1,4]-benzoquinone + NADH
-
Burkholderia cepacia AC1100 1,2,4-trihydroxybenzene + NAD+
-
r
5-chloro-2-hydroxy-[1,4]-benzoquinone + NADH
-
Burkholderia cepacia 5-chloro-1,2,4-trihydroxybenzene + NAD+
-
r
5-chloro-2-hydroxy-[1,4]-benzoquinone + NADH
-
Burkholderia cepacia AC1100 5-chloro-1,2,4-trihydroxybenzene + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 22000, SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
hydroxybenzoquinone reductase
-
Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FMN
-
Burkholderia cepacia