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Literature summary for 1.6.5.9 extracted from
- Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae (1988), Eur. J. Biochem., 176, 377-384.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| flavone | - |
Saccharomyces cerevisiae |  |
| additional information | NAD+, Mg-ADP, Mg-ATP, Ca2+ (all at 2 mM) or piericidin, rotenone (both at 0.01 mM) or adenosyl-3'-O-[3-[N-(4-azido-2-nitrophenyl)-amino]propionyl]-NAD+ (0.05 mM) are without inhibitory or stimulatory effects | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | mitochondrial inner membrane | Saccharomyces cerevisiae | 31966 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography and blue Sepharose CL-6B column chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.78 | - |
crude extract, using ubiquinone-2 as substrate, at pH 6.2, temperature not specified in the publication | Saccharomyces cerevisiae |
| 61.9 | - |
after 2134fold purification, using ubiquinone-6 as substrate, at pH 6.2, temperature not specified in the publication | Saccharomyces cerevisiae |
| 1671 | - |
after 2134fold purification, using ubiquinone-2 as substrate, at pH 6.2, temperature not specified in the publication | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Saccharomyces cerevisiae | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| NADH + H+ + 2,6-dichloroindophenol | - |
Saccharomyces cerevisiae | NAD+ + reduced 2,6-dichloroindophenol | - |
? | |
| NADH + H+ + menaquinone | - |
Saccharomyces cerevisiae | NAD+ + menaquinol | - |
? | |
| NADH + H+ + ubiquinone-10 | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol-10 | - |
? | |
| NADH + H+ + ubiquinone-2 | ubiquinone is the best electron acceptor | Saccharomyces cerevisiae | NAD+ + ubiquinol-2 | - |
? | |
| NADH + H+ + ubiquinone-6 | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol-6 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH:Q6 oxidoreductase | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
NADH | using ubiquinone-2 as substrate, in 20 mM MOPS/KOH, 1 mM EDTA, 0.03% (v/v) Triton X-100, 200 mM KCl, pH 6.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
with ubiquinone-2 as acceptor a sharp maximum at pH 6.2 is obtained | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | with ubiquinone-6 as acceptor the activity is almost constant between pH 4.5 and 9.5, at pH values lower than 4.5 and higher than 9.5, enzymic activity is irreversibly destroyed | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | non-covalently linked | Saccharomyces cerevisiae | |
| additional information | the enzyme contains no iron-sulfur clusters | Saccharomyces cerevisiae | |
| NADH | the enzyme is specific for NADH. NADPH or deamino-NADH show rates that are at least 250times lower than obtained with NADH at any pH between 5.5 and 8.5 | Saccharomyces cerevisiae | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.095 | - |
using ubiquinone-2 as substrate, in 20 mM MOPS/KOH, 1 mM EDTA, 0.03% (v/v) Triton X-100, 200 mM KCl, pH 6.2, temperature not specified in the publication | Saccharomyces cerevisiae | flavone | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | the amount of enzyme in the cell is subject to glucose repression, it increases slightly when cells, grown on glucose or lactate, enter the stationary phase | up |
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