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Literature summary for 1.7.1.1 extracted from

  • Jones, P.W.; Mhuimhneachain, M.N.
    The activity and stability of wheat nitrate reductase in vitro (1985), Phytochemistry, 24, 385-392.
No PubMed abstract available
Search for more literature for 1.7.1.1

General Stability

General Stability Organism
the decay rate of nitrate reductase activity in crude extracts is due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30% and the other in the 50-70% saturated (NH4)2SO4 fraction of the crude extract Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
202000
-
 sucrose density gradient centrifugation, gel filtration Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Triticum aestivum
-
shoot
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
-
 Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH
-
 Triticum aestivum nitrite + NAD+ + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30-50% (NH4)2SO4, 150 mM potassium phosphate buffer, pH 7.5, 0.01 mM FAD, 1 mM dithiothreitol, half-life: 36.8 min Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Triticum aestivum