Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.1 extracted from

  • Lo Piero, A.R.; Cultrone, A.; Monachello, D.; Petrone, G.
    Different kinetic and regulatory properties of soluble and membrane-bound nitrate reductases in tomato leaves (2003), Plant Sci., 165, 139-145.
No PubMed abstract available
Search for more literature for 1.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 0.1-5 mM, strong, both cytosolic and membrane-bound isozyme Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 NADH pH 7.5, 30°C, membrane-bound isoform Solanum lycopersicum
0.048
-
 NADH pH 7.5, 30°C, cytosolic isoform Solanum lycopersicum
0.27
-
 nitrate pH 7.5, 30°C, cytosolic isoform Solanum lycopersicum
0.97
-
 nitrate pH 7.5, 30°C, membrane-bound isoform Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol two isozymes, a membranebound, and a cytosolic form Solanum lycopersicum 5829
-
membrane two isozymes, a membranebound, and a cytosolic form Solanum lycopersicum 16020
-

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 cv. Mill, membrane-bound isozyme remains more active in the dark and is more easily recovered after transfer to light than cytosolic isoform, inhibition of both forms by saline stress, cytosolic form is strongly induced and strictly dependent on presence of nitrate
-

Purification (Commentary)

Purification (Comment) Organism
extracting strength of different detergents on membrane-bound isozyme Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + nitrate + H+
-
 Solanum lycopersicum NAD+ + nitrite + H2O
-
 ?