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Literature summary for 1.7.1.1 extracted from

  • Mohn, M.A.; Thaqi, B.; Fischer-Schrader, K.
    Isoform-specific NO synthesis by Arabidopsis thaliana nitrate reductase (2019), Plants (Basel), 8, 67 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 Arabidopsis thaliana
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information expression of the N-terminal fragment of NIA1, residues 1-627, i.e. NIA1-Mo-heme Arabidopsis thaliana
additional information expression of the N-terminal fragment of NIA2, residues 1-625, i.e. NIA2-Mo-heme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
0.035
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
0.443
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
2.12
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P11035 isoform Nia2
-
Arabidopsis thaliana P11832 isoform Nia1
-

Renatured (Commentary)

Renatured (Comment) Organism
recombinant expression of the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana
recombinant expression of the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally reduces nitrite, reaction of EC 1.7.1.15 Arabidopsis thaliana ?
-
-
reduced methyl viologen + nitrate
-
 Arabidopsis thaliana methyl viologen + nitrite
-
 ?

Synonyms

Synonyms Comment Organism
NIA1
-
 Arabidopsis thaliana
Nia2
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.6
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
13.4
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
51
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
69
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
155
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana