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Literature summary for extracted from

  • Roussos, G.G.; Nason, A.
    Pyridine nucleotide-nitrite and -hydroxylamine enzymes from soybean leaves (1960), J. Biol. Chem., 235, 2997-3007.
    View publication on PubMed


Inhibitors Comment Organism Structure
2,2'-dipyridyl 1 mM, 84% inhibition Glycine max
8-hydroxyquinoline 1 mM, complete inhibition Glycine max
hydrazine 1 mM, complete inhibition Glycine max
KCN 0.1 mM, complete inhibition Glycine max
NaN3 1 mM, complete inhibition Glycine max
o-phenanthroline 1 mM, complete inhibition Glycine max
p-chloromercuribenzoate 0.1 mM, complete inhibition Glycine max
potassium ethyl xanthate
Glycine max
Glycine max
Sodium diethyl dithiocarbamate 1 mM, complete inhibition Glycine max


Localization Comment Organism GeneOntology No. Textmining
Glycine max


Metals/Ions Comment Organism Structure
Mn2+ 0.1 mM, approx. 6fold stimulation Glycine max


Organism UniProt Comment Textmining
Glycine max

Purification (Commentary)

Purification (Comment) Organism
enzyme not seperated from pyridine nucleotide-nitrite reductase Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
794.1 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute Glycine max

Storage Stability

Storage Stability Organism
-20°C, 1 month, 60-90% loss of activity Glycine max
4°C, 1 week, no loss in activity Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + NADH NADH is 1.5fold more effective than NADPH Glycine max NH3 + NAD+ + H2O

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
complete loss of activity after 5 min Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.1
Glycine max


Cofactor Comment Organism Structure
additional information purified enzyme: absolute requirement for an unidentified, dissociable, heat-stable, organic factor obtained from soybean leaf extract, no flavin requirement Glycine max
Glycine max
NADPH about 65% of the activity with NADH Glycine max