Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, complete inhibition | Rattus norvegicus | |
KCN | - |
Rattus norvegicus | |
N-ethylmaleimide | 1 mM, 50% inhibition | Rattus norvegicus | |
p-chloromercuribenzoate | 1 mM, complete inhibition | Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
phosphate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | enzyme also reduces anthranilic hydroxamic acid and nicotinic hydroxamic acid, affinity is about twenty times lower than for hydroxylamine | Rattus norvegicus | NH3 + NAD+ + H2O | - |
? | |
hydroxylamine + NADH | NADH is 1.5fold more effective than NADPH | Rattus norvegicus | NH3 + NAD+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
stable for more than 1 h | Rattus norvegicus |
38 | - |
1 h, inactivation | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8.4 | pH 5.4: considerable activity in Tris-maleate buffer, pH 8.4: about 50% activity | Rattus norvegicus |
5.5 | 7.2 | pH 5.5: about 70% of activity maximum, pH 7.2: 50% activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADPH | about 65% of the activity with NADH | Rattus norvegicus |