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Literature summary for 1.7.1.10 extracted from

  • Zucker, M.; Nason, A.
    A pyridine nucleotide-hydroxylamine reductase from Neurospora (1955), J. Biol. Chem., 213, 463-478.
    View publication on PubMed

General Stability

General Stability Organism
dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity Neurospora crassa
little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Neurospora crassa
8-hydroxyquinoline
-
Neurospora crassa
Cu2+
-
Neurospora crassa
KCN 0.1 mM, complete inhibition Neurospora crassa
NaN3
-
Neurospora crassa
o-phenanthroline
-
Neurospora crassa
potassium ethyl xanthate
-
Neurospora crassa
Salicylaldoxime
-
Neurospora crassa
Sodium diethyl dithiocarbamate
-
Neurospora crassa
sulfite
-
Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NADH
-
Neurospora crassa
0.1
-
NADPH
-
Neurospora crassa
3.8
-
hydroxylamine
-
Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
360.0 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute Neurospora crassa

Storage Stability

Storage Stability Organism
-15┬░C, slow loss of activity over several weeks Neurospora crassa
4┬░C, overnight Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + NADH no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH Neurospora crassa NH3 + NAD+ + H2O
-
ir
hydroxylamine + NADH no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid Neurospora crassa NH3 + NAD+ + H2O
-
ir
hydroxylamine + NADPH + H+
-
Neurospora crassa NH3 + NADP+ + H2O
-
r
NO2- + NADH
-
Neurospora crassa NH3 + H2O + NAD+
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
50
-
5 min, stable Neurospora crassa

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 highest stability Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD stimulates Neurospora crassa
NADH
-
Neurospora crassa
NADPH
-
Neurospora crassa