Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae O1 | Q9KTK0 | - |
- |
Vibrio cholerae O1 ATCC 39315 | Q9KTK0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-cyano-7-carbaguanine + 2 NADPH + 2 H+ | - |
Vibrio cholerae O1 | 7-aminomethyl-7-carbaguanine + 2 NADP+ | - |
? | |
7-cyano-7-carbaguanine + 2 NADPH + 2 H+ | - |
Vibrio cholerae O1 ATCC 39315 | 7-aminomethyl-7-carbaguanine + 2 NADP+ | - |
? | |
additional information | the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol | Vibrio cholerae O1 | ? | - |
? | |
additional information | the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol | Vibrio cholerae O1 ATCC 39315 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent 7-cyano-7-deazaguanine reductase | - |
Vibrio cholerae O1 |
QueF | - |
Vibrio cholerae O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Vibrio cholerae O1 |