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Literature summary for 1.7.1.13 extracted from

  • Jung, J.; Braun, J.; Czabany, T.; Nidetzky, B.
    Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli (2019), Catal. Sci. Technol., 9, 842-853 .
No PubMed abstract available
Search for more literature for 1.7.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C190A the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity Escherichia coli
C190S the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity Escherichia coli
D197A the variant does not take up protons in conjunction with preQ0 binding Escherichia coli
D197H the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme Escherichia coli
H229A the variant readily forms the thioimidate adduct and is 24fold less active for preQ0 reduction than wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ Escherichia coli i.e. preQ0 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q46920
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity column chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ i.e. preQ0 Escherichia coli 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Synonyms

Synonyms Comment Organism
QueF
-
 Escherichia coli