Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.14 extracted from

  • Chao, L.Y.; Rine, J.; Marletta, M.A.
    Spectroscopic and kinetic studies of Nor1, a cytochrome P450 nitric oxide reductase from the fungal pathogen Histoplasma capsulatum (2008), Arch. Biochem. Biophys., 480, 132-137.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.14

Cloned(Commentary)

Cloned (Comment) Organism
a 404 amino acid fragment of Nor1p is expressed in Escherichia coli JM109 cells Histoplasma capsulatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NO in 100 mM potassium phosphate (pH 7.5), at 25°C Histoplasma capsulatum

Organism

Organism UniProt Comment Textmining
Histoplasma capsulatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Poros HQ anion-exchange column chromatography Histoplasma capsulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DEA-NONOate + NADH + H+
-
 Histoplasma capsulatum N2O + NAD+ + ?
-
 ?
NO + NADH + H+
-
 Histoplasma capsulatum N2O + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cytochrome P450 nitric oxide reductase
-
 Histoplasma capsulatum
Nor1p
-
 Histoplasma capsulatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.3
-
 NO in 100 mM potassium phosphate (pH 7.5), at 25°C Histoplasma capsulatum

Cofactor

Cofactor Comment Organism Structure
heme
-
 Histoplasma capsulatum
NADH Nor1p oxidizes NADH but has no activity with NADPH Histoplasma capsulatum

General Information

General Information Comment Organism
physiological function Nor1p plays a protective role against macrophage-derived nitric oxide Histoplasma capsulatum