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Literature summary for 1.7.1.15 extracted from
- Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon (1992), Mol. Microbiol., 6, 2805-2813.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JCB387 | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
x * 12000, NirD polypeptide, SDS-PAGE | Escherichia coli |
| 90000 | - |
x * 90000, NirB polypeptide, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + NADH + H+ | Escherichia coli | - |
ammonia + NAD+ + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A9I8 | NirD polypeptide | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NirB, NirD polypeptides are essential for NADH-dependent nitrite reductase activity | Escherichia coli | ? | - |
? | |
| nitrite + NADH + H+ | - |
Escherichia coli | ammonia + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 12000, NirD polypeptide, SDS-PAGE | Escherichia coli |
| ? | x * 90000, NirB polypeptide, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-dependent nitrite reductase | - |
Escherichia coli |
| nirB | - |
Escherichia coli |
| nirD | functional subunit of the active enzyme | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 30 | the nitrite reductase activities of concentrated mixtures prepared on ice do not increase during subsequent incubation at 30°C or at 4°C | Escherichia coli |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Escherichia coli | gene expression is induced during anaerobic growth | down |
| Escherichia coli | gene expression is co-ordinately repressed by oxygen | up |
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