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Literature summary for 1.7.1.16 extracted from

  • Rieble, S.; Joshi, D.K.; Gold, M.H.
    Aromatic nitroreductase from the basidiomycete Phanerochaete chrysosporium (1994), Biochem. Biophys. Res. Commun., 205, 298-304.
    View publication on PubMed
Search for more literature for 1.7.1.16

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated Phanerodontia chrysosporium 16020
-

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
membrane-associated enzyme, can be solubilized using Triton X-100 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1900
-
 cosubstrate NADH, pH 7.0, 47°C Phanerodontia chrysosporium
3600
-
 cosubstrate NADPH, pH 7.0, 47°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium 1-amino-3-nitrobenzene + 2 NADP+ + H2O about 90% 1-amino-3-nitrobenzene plus 10% 1-hydroxylamino-3-nitrobenzene ?
1-hydroxylamino-3-nitrobenzene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium 1-amino-3-nitrobenzene + 2 NADP+ + H2O
-
 ?
1-nitroso-3-nitrobenzene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium ? + 2 NADP+ + H2O only the amino product can be observed ?
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium ? + 2 NADP+ + H2O products are hydroxylamino- and amino-dinitrotoluene isomersd ?
2,4-dichloro-1-nitrobenzene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium 2,4-dichloro-N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium ? + 2 NADP+ + H2O
-
 ?
2,4-dinitrotoluene + 2 NADPH + 2 H+
-
 Phanerodontia chrysosporium ? + 2 NADP+ + H2O most abundant products are amino-nitrotoluene isomers, plus some hydroxylamino and diaminotoluene isomers ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Phanerodontia chrysosporium