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Literature summary for 1.7.1.16 extracted from

  • Somerville, C.C.; Nishino, S.F.; Spain, J.C.
    Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45 (1995), J. Bacteriol., 177, 3837-3842.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.16

Activating Compound

Activating Compound Comment Organism Structure
hydroxylamine 1 mM, 108% of initial activity Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
1-Naphthylamine 1 mM, 31% residual activity Pseudomonas oleovorans
CuSO4 0.5 mM, 78% residual activity Pseudomonas oleovorans
cysteine 1 mM, 87% residual activity Pseudomonas oleovorans
dicumarol 0.01 mM, 40% residual activity Pseudomonas oleovorans
N-ethylmaleimide 1 mM, 77% residual activity Pseudomonas oleovorans
neocuproin 1 mM, 39% residual activity Pseudomonas oleovorans
nitrite 1 mM, 77% residual activity Pseudomonas oleovorans
p-hydroxymercuribenzoate 1 mM, 46% residual activity Pseudomonas oleovorans
potassium cyanate 0.5 mM, 75% residual activity Pseudomonas oleovorans
salicyl hydroxamate 1 mM, 6% residual activity Pseudomonas oleovorans
Sodium azide 1 mM, 35% residual activity Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 nitrobenzene temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans
0.009
-
 menadione temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans
0.183
-
 NADPH temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel fitration Pseudomonas oleovorans
33000
-
 1 * 33000, SDS-PAGE Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrobenzene + 2 NADPH + 2 H+ Pseudomonas oleovorans
-
 N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
nitrobenzene + 2 NADPH + 2 H+ Pseudomonas oleovorans JS45
-
 N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
nitrosobenzene + NADPH + H+ Pseudomonas oleovorans
-
 N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?
nitrosobenzene + NADPH + H+ Pseudomonas oleovorans JS45
-
 N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans Q6DLR9
-
-
Pseudomonas oleovorans JS45 Q6DLR9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
245
-
 temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product Pseudomonas oleovorans ?
-
 ?
additional information 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product Pseudomonas oleovorans JS45 ?
-
 ?
nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans JS45 N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
nitrobenzene + menadione
-
 Pseudomonas oleovorans N-hydroxyaniline + menadiol + H2O
-
 ?
nitrobenzene + menadione
-
 Pseudomonas oleovorans JS45 N-hydroxyaniline + menadiol + H2O
-
 ?
nitrosobenzene + NADPH + H+
-
 Pseudomonas oleovorans N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?
nitrosobenzene + NADPH + H+
-
 Pseudomonas oleovorans JS45 N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
FMN tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein Pseudomonas oleovorans
additional information no cofactor: NADH Pseudomonas oleovorans
NADPH
-
 Pseudomonas oleovorans